Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23149 | 5' | -57.2 | NC_005178.1 | + | 18049 | 0.7 | 0.282284 |
Target: 5'- aCuCCAGGGCGAacuggacgguauACGGCGCgggaCGGUAg -3' miRNA: 3'- aGcGGUCCCGCU------------UGCCGUGa---GUCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 2650 | 0.66 | 0.489411 |
Target: 5'- uUCGCgaUAGGGUGccucgaucaGGCGGCGCaCAGUGa -3' miRNA: 3'- -AGCG--GUCCCGC---------UUGCCGUGaGUCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 30486 | 0.66 | 0.478924 |
Target: 5'- cUCGcCCAGGGCcg--GGCACUCAa--- -3' miRNA: 3'- -AGC-GGUCCCGcuugCCGUGAGUcaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 25679 | 0.66 | 0.46855 |
Target: 5'- -gGCCuGGGCGAucugcuCGGCGCUgUAGg-- -3' miRNA: 3'- agCGGuCCCGCUu-----GCCGUGA-GUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 16331 | 0.67 | 0.428259 |
Target: 5'- -gGCCGGccGGgGAGCGGguUUCGGUGc -3' miRNA: 3'- agCGGUC--CCgCUUGCCguGAGUCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 9368 | 0.68 | 0.345613 |
Target: 5'- gUCGCCAGGGCGc-CGGC-CgaggCGGa-- -3' miRNA: 3'- -AGCGGUCCCGCuuGCCGuGa---GUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 34830 | 0.68 | 0.337167 |
Target: 5'- cUCGcCCAGGGCGGcguucagcagcGCGGCGCg-GGUu- -3' miRNA: 3'- -AGC-GGUCCCGCU-----------UGCCGUGagUCAua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 5334 | 0.68 | 0.337167 |
Target: 5'- -gGcCCAGGGCGGcgucgAUGGCcaaacGCUCGGUGUc -3' miRNA: 3'- agC-GGUCCCGCU-----UGCCG-----UGAGUCAUA- -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 27497 | 0.7 | 0.282284 |
Target: 5'- -aGCUccaGGGGCGAugGGCGCUggccaggagcgCGGUGa -3' miRNA: 3'- agCGG---UCCCGCUugCCGUGA-----------GUCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 28127 | 0.7 | 0.275048 |
Target: 5'- -gGCCGGGGCGGuuu-CGCUCAGUGg -3' miRNA: 3'- agCGGUCCCGCUugccGUGAGUCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 19702 | 0.72 | 0.194079 |
Target: 5'- cUCGCCgAGGGCGGcaGGCGCUCccagGGUGc -3' miRNA: 3'- -AGCGG-UCCCGCUugCCGUGAG----UCAUa -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 26328 | 1.06 | 0.000524 |
Target: 5'- cUCGCCAGGGCGAACGGCACUCAGUAUg -3' miRNA: 3'- -AGCGGUCCCGCUUGCCGUGAGUCAUA- -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 28270 | 0.66 | 0.438141 |
Target: 5'- cCGCCaauggugucaguGGGGUGAcCGGCGCUgCGGg-- -3' miRNA: 3'- aGCGG------------UCCCGCUuGCCGUGA-GUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 14743 | 0.67 | 0.428259 |
Target: 5'- gUGCCc--GCGAugGGCACUCGGc-- -3' miRNA: 3'- aGCGGuccCGCUugCCGUGAGUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 23353 | 0.67 | 0.408895 |
Target: 5'- aUCGgcaCCGGGGCGucgcaccagGGCGGCAgUCGGg-- -3' miRNA: 3'- -AGC---GGUCCCGC---------UUGCCGUgAGUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 28122 | 0.67 | 0.399421 |
Target: 5'- -gGCCuGGGCGGuggcaccgaugGCGGCGCcCAGg-- -3' miRNA: 3'- agCGGuCCCGCU-----------UGCCGUGaGUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 23458 | 0.68 | 0.362959 |
Target: 5'- -gGCCAGGGCGccCGGCGCgaagcCGGc-- -3' miRNA: 3'- agCGGUCCCGCuuGCCGUGa----GUCaua -5' |
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23149 | 5' | -57.2 | NC_005178.1 | + | 29064 | 0.68 | 0.354211 |
Target: 5'- uUCGCCugauGGGCGGcACGGCGUUCAa--- -3' miRNA: 3'- -AGCGGu---CCCGCU-UGCCGUGAGUcaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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