Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23150 | 5' | -61 | NC_005178.1 | + | 27455 | 0.66 | 0.31963 |
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Target: 5'- cGGCgGCCCAGCgcaucaGCC-UGGAccagaagauCGCCg -3' miRNA: 3'- -CCGgCGGGUUGac----CGGuACCU---------GCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 33429 | 0.66 | 0.326701 |
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Target: 5'- cGCCGucguagacccacaCCCuGCUGGCguCGUGGAUGUCg -3' miRNA: 3'- cCGGC-------------GGGuUGACCG--GUACCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 6510 | 0.66 | 0.327494 |
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Target: 5'- cGCCGCCaccgugcgcaugCggUUGGCCAagGGaAUGCCUc -3' miRNA: 3'- cCGGCGG------------GuuGACCGGUa-CC-UGCGGA- -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 11758 | 0.66 | 0.327494 |
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Target: 5'- cGGCCgugGCCCGA---GCCAUGGGgguucCGCCg -3' miRNA: 3'- -CCGG---CGGGUUgacCGGUACCU-----GCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 8833 | 0.66 | 0.327494 |
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Target: 5'- cGCCGCCCuuGCcGGCCcacauGUcGGcCGCCa -3' miRNA: 3'- cCGGCGGGu-UGaCCGG-----UA-CCuGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 15284 | 0.66 | 0.327494 |
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Target: 5'- cGCCGCCUGGCgGGCCAgccUGGcgguCGgCUu -3' miRNA: 3'- cCGGCGGGUUGaCCGGU---ACCu---GCgGA- -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 34567 | 0.66 | 0.327494 |
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Target: 5'- cGCaCGCUCGGCaGGCCAUccgcgcucGGGCgGCCg -3' miRNA: 3'- cCG-GCGGGUUGaCCGGUA--------CCUG-CGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 3722 | 0.66 | 0.3355 |
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Target: 5'- uGGaCCGCCaggccaAGCUGGCCAccuucaagcUGacGCGCCg -3' miRNA: 3'- -CC-GGCGGg-----UUGACCGGU---------ACc-UGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 1035 | 0.66 | 0.3355 |
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Target: 5'- cGGCCGuCCCAuc--GCCAcuagcaaaucuUGGACGCgCUg -3' miRNA: 3'- -CCGGC-GGGUugacCGGU-----------ACCUGCG-GA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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