Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23150 | 5' | -61 | NC_005178.1 | + | 32441 | 0.71 | 0.150044 |
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Target: 5'- gGGCCGgaaCCCAGCUcagcacgcGGCCAUGGAaGCg- -3' miRNA: 3'- -CCGGC---GGGUUGA--------CCGGUACCUgCGga -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 33429 | 0.66 | 0.326701 |
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Target: 5'- cGCCGucguagacccacaCCCuGCUGGCguCGUGGAUGUCg -3' miRNA: 3'- cCGGC-------------GGGuUGACCG--GUACCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 33792 | 0.67 | 0.282446 |
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Target: 5'- uGCCGCCCuuggcgaUGGCCcgGcGCGUCa -3' miRNA: 3'- cCGGCGGGuug----ACCGGuaCcUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 34034 | 0.66 | 0.30433 |
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Target: 5'- cGuuGUCCAcguagaagaUGGCCG-GGACGCCg -3' miRNA: 3'- cCggCGGGUug-------ACCGGUaCCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 34567 | 0.66 | 0.327494 |
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Target: 5'- cGCaCGCUCGGCaGGCCAUccgcgcucGGGCgGCCg -3' miRNA: 3'- cCG-GCGGGUUGaCCGGUA--------CCUG-CGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 34639 | 0.7 | 0.167321 |
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Target: 5'- uGCgGCUCGAcCUGGCCGaGGcGCGCCa -3' miRNA: 3'- cCGgCGGGUU-GACCGGUaCC-UGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 34984 | 0.71 | 0.145986 |
|
Target: 5'- uGGCCGC---GCUGGCCGaGG-CGCCg -3' miRNA: 3'- -CCGGCGgguUGACCGGUaCCuGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 35379 | 0.66 | 0.302084 |
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Target: 5'- cGGCUGCugacgaacaCCAGCgagGGCCAacuggccccggcgcUGGcCGCCa -3' miRNA: 3'- -CCGGCG---------GGUUGa--CCGGU--------------ACCuGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 36113 | 0.69 | 0.19141 |
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Target: 5'- uGCCGCCCAgguuggGCUGGCgGgugcaGGugGCg- -3' miRNA: 3'- cCGGCGGGU------UGACCGgUa----CCugCGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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