Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23150 | 5' | -61 | NC_005178.1 | + | 14253 | 0.67 | 0.257854 |
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Target: 5'- aGGCgaGCCCuGGCgcuguucgucaagggUGGCCAgaucggGGACGCCc -3' miRNA: 3'- -CCGg-CGGG-UUG---------------ACCGGUa-----CCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 25443 | 0.67 | 0.255232 |
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Target: 5'- cGGCgCGCCCAuc-GGCCA-GGccCGCCa -3' miRNA: 3'- -CCG-GCGGGUugaCCGGUaCCu-GCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 22856 | 0.68 | 0.242451 |
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Target: 5'- gGGCUGCCCAuCcGGCCAgcagaUGaucGCGCCg -3' miRNA: 3'- -CCGGCGGGUuGaCCGGU-----ACc--UGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 14077 | 0.69 | 0.212822 |
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Target: 5'- cGGCCGagCAGCgGGCCGgcuucgcgccGGGCGCCc -3' miRNA: 3'- -CCGGCggGUUGaCCGGUa---------CCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 18525 | 0.69 | 0.207283 |
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Target: 5'- --gUGCCCGACUGGaUCA-GGACGCUg -3' miRNA: 3'- ccgGCGGGUUGACC-GGUaCCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 14182 | 0.69 | 0.207283 |
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Target: 5'- -aUCGCCCGACUGccGCCcugGUGcGACGCCc -3' miRNA: 3'- ccGGCGGGUUGAC--CGG---UAC-CUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 3643 | 0.69 | 0.207283 |
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Target: 5'- aGGCCGCCgAGCaucgccGCCAggcgcggcaGGACGCCc -3' miRNA: 3'- -CCGGCGGgUUGac----CGGUa--------CCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 17127 | 0.69 | 0.201869 |
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Target: 5'- aGGCCgaggguuucGCgCAGCuUGGCCAgguUGGuCGCCUg -3' miRNA: 3'- -CCGG---------CGgGUUG-ACCGGU---ACCuGCGGA- -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 14736 | 0.86 | 0.009314 |
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Target: 5'- cGGUCGCUCAGCUGGCgGaUGGACGCCUc -3' miRNA: 3'- -CCGGCGGGUUGACCGgU-ACCUGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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