Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23150 | 5' | -61 | NC_005178.1 | + | 3064 | 0.68 | 0.230206 |
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Target: 5'- cGCCGCCCGGacaUGagcguucCCGacUGGGCGCCUg -3' miRNA: 3'- cCGGCGGGUUg--ACc------GGU--ACCUGCGGA- -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 4532 | 0.68 | 0.248773 |
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Target: 5'- cGGCCuGCCCAucgaGCC--GGACGCCg -3' miRNA: 3'- -CCGG-CGGGUugacCGGuaCCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 15261 | 0.67 | 0.255232 |
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Target: 5'- cGGCCGCagaccaCGACgaUGGCCcc-GACGCCc -3' miRNA: 3'- -CCGGCGg-----GUUG--ACCGGuacCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 28315 | 0.67 | 0.255232 |
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Target: 5'- gGGCCGCUgGAgUGGC--UGGugGCg- -3' miRNA: 3'- -CCGGCGGgUUgACCGguACCugCGga -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 12098 | 0.67 | 0.268561 |
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Target: 5'- uGGUCGCgUGGCgggccUGGCCgAUGGGCGCg- -3' miRNA: 3'- -CCGGCGgGUUG-----ACCGG-UACCUGCGga -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 28980 | 0.67 | 0.268561 |
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Target: 5'- uGCgCGCCC--CUGGCC-UGGGCGgCa -3' miRNA: 3'- cCG-GCGGGuuGACCGGuACCUGCgGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 28552 | 0.67 | 0.275434 |
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Target: 5'- gGGCCGUCC-GCcGcGCCAccggGGGCGUCg -3' miRNA: 3'- -CCGGCGGGuUGaC-CGGUa---CCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 16592 | 0.67 | 0.282446 |
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Target: 5'- -aCCGCCC--CUGGUCAagcUGGGCGUCc -3' miRNA: 3'- ccGGCGGGuuGACCGGU---ACCUGCGGa -5' |
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| 23150 | 5' | -61 | NC_005178.1 | + | 16414 | 0.66 | 0.31963 |
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Target: 5'- aGGUgCGCCaugaccuACUGGCUgcGGAUGCCc -3' miRNA: 3'- -CCG-GCGGgu-----UGACCGGuaCCUGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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