Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23152 | 3' | -60.7 | NC_005178.1 | + | 8167 | 0.66 | 0.298079 |
Target: 5'- aUAGCAGCuGCGCUcguagGGgCUGCUGAuGCc- -3' miRNA: 3'- -GUCGUCG-CGCGG-----CCgGAUGACU-CGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 4120 | 0.66 | 0.298079 |
Target: 5'- cCAGCAGgGUGUgGGUCUACgacGGCg- -3' miRNA: 3'- -GUCGUCgCGCGgCCGGAUGac-UCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 14852 | 0.66 | 0.295073 |
Target: 5'- uCGGCAGCGacccggcgauccaGCuCGGCCUGggcgaggucgccCUGGGCUu -3' miRNA: 3'- -GUCGUCGCg------------CG-GCCGGAU------------GACUCGAu -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 11336 | 0.66 | 0.290609 |
Target: 5'- gAGcCAGCG-GUCGGCCUGCUGuucGUUu -3' miRNA: 3'- gUC-GUCGCgCGGCCGGAUGACu--CGAu -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 23463 | 0.66 | 0.28987 |
Target: 5'- gGGCgcccGGCGCgaaGCCGGCCcGCUGcucggccGGCUGc -3' miRNA: 3'- gUCG----UCGCG---CGGCCGGaUGAC-------UCGAU- -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 27735 | 0.66 | 0.283286 |
Target: 5'- gCAGCAGCGC-CUGGaCCUGgcGAGCc- -3' miRNA: 3'- -GUCGUCGCGcGGCC-GGAUgaCUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 11142 | 0.66 | 0.283286 |
Target: 5'- uCAGCGcGgGCGCCcuGGCCgccgccCUGGGCUu -3' miRNA: 3'- -GUCGU-CgCGCGG--CCGGau----GACUCGAu -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 4520 | 0.66 | 0.27611 |
Target: 5'- gCGGCAaCGCuCCGGCCUGCccaucGAGCc- -3' miRNA: 3'- -GUCGUcGCGcGGCCGGAUGa----CUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 22444 | 0.67 | 0.262196 |
Target: 5'- cCAGCuGCGCGUCGaGuCCUGC-GAGUUc -3' miRNA: 3'- -GUCGuCGCGCGGC-C-GGAUGaCUCGAu -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 32915 | 0.67 | 0.248857 |
Target: 5'- cCAGCGGCGUggucGCCGGCaUGCcGAugGCUGg -3' miRNA: 3'- -GUCGUCGCG----CGGCCGgAUGaCU--CGAU- -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 26452 | 0.67 | 0.248857 |
Target: 5'- uCAGCAGCGCggcgcuGCCGGCCgaggugGAuGCg- -3' miRNA: 3'- -GUCGUCGCG------CGGCCGGauga--CU-CGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 33924 | 0.67 | 0.2424 |
Target: 5'- uCGGCGGCcgGUGCCGGCUUuccgGGGCg- -3' miRNA: 3'- -GUCGUCG--CGCGGCCGGAuga-CUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 30274 | 0.67 | 0.2424 |
Target: 5'- aCAGCGGCgGCGgCaGCgUGCUGcGGCUAg -3' miRNA: 3'- -GUCGUCG-CGCgGcCGgAUGAC-UCGAU- -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 20714 | 0.67 | 0.2424 |
Target: 5'- gAGCGGCGCGaccaggcuaUCGGCCUGguuuugcaugucCUGGGCc- -3' miRNA: 3'- gUCGUCGCGC---------GGCCGGAU------------GACUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 21795 | 0.67 | 0.23236 |
Target: 5'- gCAGCAGCucguggagauacuccGCGUCGGCCUucuCUGcGGCc- -3' miRNA: 3'- -GUCGUCG---------------CGCGGCCGGAu--GAC-UCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 7502 | 0.68 | 0.206548 |
Target: 5'- aGGUgauAGCGCGUgaaUGGCCcggcGCUGAGCUGc -3' miRNA: 3'- gUCG---UCGCGCG---GCCGGa---UGACUCGAU- -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 35494 | 0.68 | 0.195657 |
Target: 5'- aCGGUAGCGCGUacccggcgaaGGUCUACgGGGCg- -3' miRNA: 3'- -GUCGUCGCGCGg---------CCGGAUGaCUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 28282 | 0.7 | 0.148459 |
Target: 5'- uCAGUGGgGUGaCCGGCgCUGCggGAGCUGc -3' miRNA: 3'- -GUCGUCgCGC-GGCCG-GAUGa-CUCGAU- -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 24900 | 0.73 | 0.086301 |
Target: 5'- -cGcCAGCGCGCCGGCC-GCcGAGCc- -3' miRNA: 3'- guC-GUCGCGCGGCCGGaUGaCUCGau -5' |
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23152 | 3' | -60.7 | NC_005178.1 | + | 14082 | 0.74 | 0.07682 |
Target: 5'- gAGCAGCGgGCCGGCUUcgcGCcGGGCg- -3' miRNA: 3'- gUCGUCGCgCGGCCGGA---UGaCUCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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