Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23164 | 5' | -53.1 | NC_005178.1 | + | 4084 | 0.66 | 0.72445 |
Target: 5'- gCGACCaggUGGcCGUgggCUACGACAUcCACg -3' miRNA: 3'- -GCUGG---ACCaGCA---GAUGCUGUAcGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 4743 | 0.69 | 0.532266 |
Target: 5'- cCGGCCUGGUCugcuUCUACGGCccGUccgGCUu -3' miRNA: 3'- -GCUGGACCAGc---AGAUGCUGuaCG---UGA- -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 5525 | 0.67 | 0.645452 |
Target: 5'- gCGACCagggucaGGUCGUCgGCGugGgucgGCACc -3' miRNA: 3'- -GCUGGa------CCAGCAGaUGCugUa---CGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 12256 | 0.67 | 0.650022 |
Target: 5'- uCGACCUGGUCcgcacccugcugaagGUCaGCGcGgAUGCGCa -3' miRNA: 3'- -GCUGGACCAG---------------CAGaUGC-UgUACGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 12679 | 0.74 | 0.279817 |
Target: 5'- uGGCCUGGUUGcCUGCGGCcUGCucGCg -3' miRNA: 3'- gCUGGACCAGCaGAUGCUGuACG--UGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 14348 | 0.67 | 0.668268 |
Target: 5'- uGACCUGGUgGUCggcgACGACcUGgAg- -3' miRNA: 3'- gCUGGACCAgCAGa---UGCUGuACgUga -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 16372 | 0.66 | 0.75707 |
Target: 5'- aCGGUCUGGUCGUCcACGAUGUagGCGg- -3' miRNA: 3'- -GCUGGACCAGCAGaUGCUGUA--CGUga -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 22789 | 0.68 | 0.611157 |
Target: 5'- cCGACCUGGUCGagcagUACGGCGagGCGu- -3' miRNA: 3'- -GCUGGACCAGCag---AUGCUGUa-CGUga -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 26672 | 0.66 | 0.745235 |
Target: 5'- uGACCUucgcgaaGG-CGUCcuCGACGUGCGCc -3' miRNA: 3'- gCUGGA-------CCaGCAGauGCUGUACGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 28889 | 0.66 | 0.746318 |
Target: 5'- aGACCUGGUCGcggCUG-GGCAgGC-CUa -3' miRNA: 3'- gCUGGACCAGCa--GAUgCUGUaCGuGA- -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 29394 | 0.74 | 0.287243 |
Target: 5'- aCGACCUGGUCGUCcccacggGCGAUcucgaugGCGCc -3' miRNA: 3'- -GCUGGACCAGCAGa------UGCUGua-----CGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 30812 | 0.76 | 0.238446 |
Target: 5'- uCGACCUGGgccggCGUCUACG-CG-GCGCa -3' miRNA: 3'- -GCUGGACCa----GCAGAUGCuGUaCGUGa -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 32651 | 1.09 | 0.001181 |
Target: 5'- uCGACCUGGUCGUCUACGACAUGCACUa -3' miRNA: 3'- -GCUGGACCAGCAGAUGCUGUACGUGA- -5' |
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23164 | 5' | -53.1 | NC_005178.1 | + | 33587 | 0.68 | 0.634018 |
Target: 5'- aCGugCUGGUCG-CgaccgGCGACGcgcucGCGCUc -3' miRNA: 3'- -GCugGACCAGCaGa----UGCUGUa----CGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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