miRNA display CGI


Results 21 - 23 of 23 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23170 3' -55.2 NC_005178.1 + 10091 0.69 0.410999
Target:  5'- uGGcCCGGCGC--AUGUuccagGUCGAGCGGg -3'
miRNA:   3'- uCCuGGUCGCGcuUACG-----UAGCUCGUC- -5'
23170 3' -55.2 NC_005178.1 + 31232 0.69 0.382612
Target:  5'- cGGACUGGgGCGAcaGCAucgUCGAGCGc -3'
miRNA:   3'- uCCUGGUCgCGCUuaCGU---AGCUCGUc -5'
23170 3' -55.2 NC_005178.1 + 33887 0.71 0.321634
Target:  5'- gAGGACCAGgGCGuccUGCcgCGccuGGCGGc -3'
miRNA:   3'- -UCCUGGUCgCGCuu-ACGuaGC---UCGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.