Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23170 | 5' | -60.1 | NC_005178.1 | + | 33084 | 0.66 | 0.35688 |
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Target: 5'- gCUggGCGGGAGcGGU--CGGCAGGgUGa -3' miRNA: 3'- aGGa-CGCCCUC-CCGuaGCCGUUCgAC- -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 8072 | 0.66 | 0.323844 |
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Target: 5'- cCCaGCGGGAGGaGCGUCcGauuGCUGu -3' miRNA: 3'- aGGaCGCCCUCC-CGUAGcCguuCGAC- -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 26603 | 0.67 | 0.293141 |
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Target: 5'- ---cGCGGG-GGGCAggaGGCGGGCc- -3' miRNA: 3'- aggaCGCCCuCCCGUag-CCGUUCGac -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 35800 | 0.67 | 0.28583 |
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Target: 5'- uUCCUcGCGGGuccgcucaaGGcGGCccaguUCGGCGAGCa- -3' miRNA: 3'- -AGGA-CGCCC---------UC-CCGu----AGCCGUUCGac -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 21032 | 0.67 | 0.271639 |
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Target: 5'- aCCagGCGGG-GGGCG-CGGCGuGCg- -3' miRNA: 3'- aGGa-CGCCCuCCCGUaGCCGUuCGac -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 14922 | 0.71 | 0.150762 |
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Target: 5'- aUCCUgGUGGGAGGGUucaAUCGGgagaCAGGCg- -3' miRNA: 3'- -AGGA-CGCCCUCCCG---UAGCC----GUUCGac -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 33036 | 0.71 | 0.146636 |
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Target: 5'- aUCCUggGCGGcaccGAGGGCAUucCGGCAGGUg- -3' miRNA: 3'- -AGGA--CGCC----CUCCCGUA--GCCGUUCGac -5' |
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| 23170 | 5' | -60.1 | NC_005178.1 | + | 37523 | 1.08 | 0.000187 |
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Target: 5'- uUCCUGCGGGAGGGCAUCGGCAAGCUGa -3' miRNA: 3'- -AGGACGCCCUCCCGUAGCCGUUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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