miRNA: 3'- aC-CUGGAaCG-GCUGAAGACGGcUCGC- -5'
miRNA: 3'- aCCugGa------ACGGCUgAAGa--------CGGCUCGC- -5'
miRNA: 3'- aCCUGgaACGGCUGa-AGACGGCuCGC- -5'
miRNA: 3'- -ACCUGGAACGGCUGAAGACGGCUCGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23185 | 3' | -56.9 | NC_005259.1 | + | 9771 | 0.73 | 0.278717 |
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Target: 5'- cGaGACCUgGCuCGACUUCUGCCcGGUGu -3' miRNA: 3'- aC-CUGGAaCG-GCUGAAGACGGcUCGC- -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 14531 | 0.74 | 0.258658 |
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Target: 5'- cGGugCguuucggUGCCGAgUUCguccggcagGCCGAGCGg -3' miRNA: 3'- aCCugGa------ACGGCUgAAGa--------CGGCUCGC- -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 34697 | 0.76 | 0.200233 |
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Target: 5'- cGGAUgaUGCCGACcaUCUGCCGcGCGa -3' miRNA: 3'- aCCUGgaACGGCUGa-AGACGGCuCGC- -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 68506 | 1.09 | 0.000891 |
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Target: 5'- cUGGACCUUGCCGACUUCUGCCGAGCGa -3' miRNA: 3'- -ACCUGGAACGGCUGAAGACGGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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