miRNA: 3'- aCC-------UGGAACGGCUgaaGACGGCU--CGC- -5'
miRNA: 3'- aCCUGGaaCGGCUGaaGa---CGG-CUCGC- -5'
miRNA: 3'- aCCUGGaa-----CGGCU-Ga-AGa-CGGCUCGc -5'
miRNA: 3'- aCCUGGaa-CGGCUGaaga--CGGC---UCGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23185 | 3' | -56.9 | NC_005259.1 | + | 8306 | 0.68 | 0.585255 |
|
Target: 5'- cGGaaacgccGCCUUGUCGGag-CUGCCGAcgGCGa -3' miRNA: 3'- aCC-------UGGAACGGCUgaaGACGGCU--CGC- -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 7856 | 0.67 | 0.62922 |
|
Target: 5'- cGGACCgcGCCGACccCggugGCCcGAGUGc -3' miRNA: 3'- aCCUGGaaCGGCUGaaGa---CGG-CUCGC- -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 4741 | 0.66 | 0.682819 |
|
Target: 5'- aGGACCggcucagGCCGGuCccUCggGCCGGGCu -3' miRNA: 3'- aCCUGGaa-----CGGCU-Ga-AGa-CGGCUCGc -5' |
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| 23185 | 3' | -56.9 | NC_005259.1 | + | 3072 | 0.67 | 0.639973 |
|
Target: 5'- cGGGCCgagGCCGACaagagaGCCGcgcAGCGa -3' miRNA: 3'- aCCUGGaa-CGGCUGaaga--CGGC---UCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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