Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23185 | 5' | -56.5 | NC_005259.1 | + | 51662 | 0.66 | 0.730857 |
Target: 5'- uGCCGCGCGggCG-CGUCGGGcacGGg-- -3' miRNA: 3'- -CGGUGCGCa-GCuGUAGCCCca-CCaac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 3248 | 0.66 | 0.720595 |
Target: 5'- cGUCAUGacgagGUCGGCAUCcugcucGGGUGGUUu -3' miRNA: 3'- -CGGUGCg----CAGCUGUAGc-----CCCACCAAc -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 16645 | 0.66 | 0.720595 |
Target: 5'- gGCgACGcCGcCGACAauuUCGGGGUGa--- -3' miRNA: 3'- -CGgUGC-GCaGCUGU---AGCCCCACcaac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 42022 | 0.66 | 0.707133 |
Target: 5'- aUCACGCacgaggguugucggGUCGACugcgcuguccUCGGGGUGGgcGg -3' miRNA: 3'- cGGUGCG--------------CAGCUGu---------AGCCCCACCaaC- -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 5046 | 0.67 | 0.689358 |
Target: 5'- cGCCACGUugaucacgGUCGAgAUCGuGGGccgGGUc- -3' miRNA: 3'- -CGGUGCG--------CAGCUgUAGC-CCCa--CCAac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 51398 | 0.67 | 0.689358 |
Target: 5'- cCCACGUGUUcACAUCGccgaGGGgccGGUUGa -3' miRNA: 3'- cGGUGCGCAGcUGUAGC----CCCa--CCAAC- -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 31509 | 0.67 | 0.645971 |
Target: 5'- aCCGCGCcg-GACuugcuugAUCGGGGUGGUg- -3' miRNA: 3'- cGGUGCGcagCUG-------UAGCCCCACCAac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 29941 | 0.68 | 0.636398 |
Target: 5'- cGCCACGCGgcccggucgCGGCuggUGGGGcGGgcGg -3' miRNA: 3'- -CGGUGCGCa--------GCUGua-GCCCCaCCaaC- -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 33329 | 0.68 | 0.625758 |
Target: 5'- gGUCACGCGgaaGugAUCGGGGUcGa-- -3' miRNA: 3'- -CGGUGCGCag-CugUAGCCCCAcCaac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 67406 | 0.68 | 0.604502 |
Target: 5'- uGUCACGgGcCGACAccaUCGGGGUGc--- -3' miRNA: 3'- -CGGUGCgCaGCUGU---AGCCCCACcaac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 61877 | 0.69 | 0.583334 |
Target: 5'- uGCCGCGUGaucacgaccUCGAUAUCaGGGGUuaGGUc- -3' miRNA: 3'- -CGGUGCGC---------AGCUGUAG-CCCCA--CCAac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 34715 | 0.7 | 0.489759 |
Target: 5'- uGCCGCGCGagCGccuCGUCGGGGaaaccguUGGUg- -3' miRNA: 3'- -CGGUGCGCa-GCu--GUAGCCCC-------ACCAac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 36529 | 0.7 | 0.48086 |
Target: 5'- cCCGCGCGgugUGACAcgCGGGGUGa--- -3' miRNA: 3'- cGGUGCGCa--GCUGUa-GCCCCACcaac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 55307 | 0.74 | 0.300323 |
Target: 5'- gGCCGCGUG-CGACGaccacaGGGGUGGUcuUGg -3' miRNA: 3'- -CGGUGCGCaGCUGUag----CCCCACCA--AC- -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 28229 | 0.83 | 0.076298 |
Target: 5'- cGCCAC-CGUCGGCggCGGGGUGGUc- -3' miRNA: 3'- -CGGUGcGCAGCUGuaGCCCCACCAac -5' |
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23185 | 5' | -56.5 | NC_005259.1 | + | 68540 | 1.11 | 0.000871 |
Target: 5'- cGCCACGCGUCGACAUCGGGGUGGUUGa -3' miRNA: 3'- -CGGUGCGCAGCUGUAGCCCCACCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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