Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23187 | 5' | -51.3 | NC_005259.1 | + | 65847 | 0.66 | 0.929125 |
Target: 5'- -cGUGGgacua--CACGGUGAUGCCCg -3' miRNA: 3'- uuUACCacagcacGUGCUACUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 62337 | 0.68 | 0.857746 |
Target: 5'- ---gGGUGUcCGUgacGCGCGAgc-UGCCCg -3' miRNA: 3'- uuuaCCACA-GCA---CGUGCUacuACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 43063 | 0.67 | 0.903904 |
Target: 5'- ---cGGUGa-GcGCACGccGGUGCCCg -3' miRNA: 3'- uuuaCCACagCaCGUGCuaCUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 41603 | 0.68 | 0.849151 |
Target: 5'- ---cGGUGUCGgcaGCgGCGGcagcucGGUGCCCg -3' miRNA: 3'- uuuaCCACAGCa--CG-UGCUa-----CUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 35289 | 0.67 | 0.889594 |
Target: 5'- ---cGGUGgaCGgauucUGCGCGAUGAUGUCg -3' miRNA: 3'- uuuaCCACa-GC-----ACGUGCUACUACGGg -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 31496 | 0.68 | 0.831257 |
Target: 5'- ---cGGUGa---GCACGAUGcUGCCCg -3' miRNA: 3'- uuuaCCACagcaCGUGCUACuACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 30052 | 0.68 | 0.866917 |
Target: 5'- ---cGGUGcCGcugccgccgagagccUGCACGAUGGcaucgcggauacccUGCCCa -3' miRNA: 3'- uuuaCCACaGC---------------ACGUGCUACU--------------ACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 28182 | 0.69 | 0.812491 |
Target: 5'- ---cGGgGUCGacgcGCACGAugggccgcguguUGAUGCCCu -3' miRNA: 3'- uuuaCCaCAGCa---CGUGCU------------ACUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 12617 | 0.66 | 0.910639 |
Target: 5'- ---cGGgccgcGUCG-GCugaucgucuCGAUGGUGCCCg -3' miRNA: 3'- uuuaCCa----CAGCaCGu--------GCUACUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 2296 | 1.11 | 0.002211 |
Target: 5'- aAAAUGGUGUCGUGCACGAUGAUGCCCg -3' miRNA: 3'- -UUUACCACAGCACGUGCUACUACGGG- -5' |
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23187 | 5' | -51.3 | NC_005259.1 | + | 1837 | 0.76 | 0.39865 |
Target: 5'- cGAUGGUGgCGgccaUGCcgcCGAUGAUGCCCg -3' miRNA: 3'- uUUACCACaGC----ACGu--GCUACUACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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