Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23188 | 3' | -58.4 | NC_005259.1 | + | 41176 | 0.66 | 0.593146 |
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Target: 5'- uGGCucGAcuUCUGCGCGaGGUA-GGCCGCg -3' miRNA: 3'- gUCG--CU--AGACGCGCaCCGUgUCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 42161 | 0.66 | 0.561193 |
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Target: 5'- gGGCGGUCUGCuCG-GGCAgGauGCCGa -3' miRNA: 3'- gUCGCUAGACGcGCaCCGUgU--CGGUg -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 42817 | 0.67 | 0.528692 |
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Target: 5'- -uGCGAUCuccagaaUGUGCGgcaGCAUGGCCGCc -3' miRNA: 3'- guCGCUAG-------ACGCGCac-CGUGUCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 45397 | 0.67 | 0.498936 |
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Target: 5'- aCGGCaGUUcccCGCGUGGCGgCAGCCAg -3' miRNA: 3'- -GUCGcUAGac-GCGCACCGU-GUCGGUg -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 45865 | 0.66 | 0.582453 |
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Target: 5'- gCAGCGAUgUGa-CGUGGUGCgAGaCCACc -3' miRNA: 3'- -GUCGCUAgACgcGCACCGUG-UC-GGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 50487 | 0.68 | 0.449505 |
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Target: 5'- uCAGCGAUCUGCccgccgauuucgGCaaGGCAgAGCC-Cg -3' miRNA: 3'- -GUCGCUAGACG------------CGcaCCGUgUCGGuG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 62449 | 0.68 | 0.45918 |
|
Target: 5'- gAGCGGUgaagCUGUGCGcGGCGugucaGGCCGCc -3' miRNA: 3'- gUCGCUA----GACGCGCaCCGUg----UCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 62809 | 0.68 | 0.449505 |
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Target: 5'- gGGUGAUCUGagcaGCGUGaGCGgggugAGCCGCc -3' miRNA: 3'- gUCGCUAGACg---CGCAC-CGUg----UCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 68812 | 0.66 | 0.571799 |
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Target: 5'- uCGGUcAUCUaugGCGCGUGGCGCGaCgGCa -3' miRNA: 3'- -GUCGcUAGA---CGCGCACCGUGUcGgUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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