Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23189 | 3' | -57.9 | NC_005259.1 | + | 23821 | 0.66 | 0.610757 |
Target: 5'- uUGCGCGCC-GA-GGU-CAGCGCGg- -3' miRNA: 3'- cACGUGCGGaCUaCCAcGUCGCGUgc -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 207 | 0.66 | 0.600001 |
Target: 5'- -cGUACGCCggGgcGGUGuCGGUGCcCGg -3' miRNA: 3'- caCGUGCGGa-CuaCCAC-GUCGCGuGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 53803 | 0.66 | 0.578577 |
Target: 5'- cGUGCAcCGCaUGAUGcUGUGGCGCuCGg -3' miRNA: 3'- -CACGU-GCGgACUACcACGUCGCGuGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 33796 | 0.66 | 0.578577 |
Target: 5'- cGUGC-CGCCUGAg---GCGGCGaggaACGu -3' miRNA: 3'- -CACGuGCGGACUaccaCGUCGCg---UGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 52693 | 0.67 | 0.536298 |
Target: 5'- cGUGCACGUgUGAUGuGgGCGGauacugcccgaUGCACGg -3' miRNA: 3'- -CACGUGCGgACUAC-CaCGUC-----------GCGUGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 23377 | 0.67 | 0.525889 |
Target: 5'- aUGCcCGCCggGccGG-GCAGCGCGCc -3' miRNA: 3'- cACGuGCGGa-CuaCCaCGUCGCGUGc -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 2315 | 0.68 | 0.47511 |
Target: 5'- cGUGC-CGCCcaUGAggucgaaaaUGGUGUcGUGCACGa -3' miRNA: 3'- -CACGuGCGG--ACU---------ACCACGuCGCGUGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 47766 | 0.69 | 0.436311 |
Target: 5'- -aGUACGCCUcg----GCAGCGCGCGg -3' miRNA: 3'- caCGUGCGGAcuaccaCGUCGCGUGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 27597 | 0.69 | 0.436311 |
Target: 5'- -gGCGCGCCgcucggGGUGCccacgauGUGCACGg -3' miRNA: 3'- caCGUGCGGacua--CCACGu------CGCGUGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 44735 | 0.69 | 0.430649 |
Target: 5'- cGUGCACGCguggcuggaagcgcuCgaccucggGAUGGUGCuGCuGCGCGa -3' miRNA: 3'- -CACGUGCG---------------Ga-------CUACCACGuCG-CGUGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 24589 | 0.7 | 0.373063 |
Target: 5'- gGUGCcCGCCUGAccG-GCAGCGC-CGa -3' miRNA: 3'- -CACGuGCGGACUacCaCGUCGCGuGC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 46847 | 0.7 | 0.347929 |
Target: 5'- cGUGCGCGCCgaaAUGGaUGaccaCAGCGCGCc -3' miRNA: 3'- -CACGUGCGGac-UACC-AC----GUCGCGUGc -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 59178 | 0.74 | 0.217293 |
Target: 5'- -cGCAcCGCCg---GGUGCGGCGCugGc -3' miRNA: 3'- caCGU-GCGGacuaCCACGUCGCGugC- -5' |
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23189 | 3' | -57.9 | NC_005259.1 | + | 2914 | 1.08 | 0.000783 |
Target: 5'- aGUGCACGCCUGAUGGUGCAGCGCACGc -3' miRNA: 3'- -CACGUGCGGACUACCACGUCGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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