Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23190 | 3' | -54.3 | NC_005259.1 | + | 7497 | 0.66 | 0.851648 |
Target: 5'- -uUGAGgCGcuCGGUGUGCCGG-UCGAc -3' miRNA: 3'- ccACUCgGUu-GCCACAUGGUCuGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 19441 | 0.66 | 0.825682 |
Target: 5'- --cGGGCUGuucguCGGUcGUgGCCAGACCGGc -3' miRNA: 3'- ccaCUCGGUu----GCCA-CA-UGGUCUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 62446 | 0.66 | 0.825682 |
Target: 5'- cGGUGaAGCUGugcGCGGcGUGUCAGGCCGc -3' miRNA: 3'- -CCAC-UCGGU---UGCCaCAUGGUCUGGCu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 35121 | 0.66 | 0.816621 |
Target: 5'- cGGUGAGCaCAcGCGG-GaACCacccgcgcgacGGGCCGAg -3' miRNA: 3'- -CCACUCG-GU-UGCCaCaUGG-----------UCUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 18836 | 0.66 | 0.815705 |
Target: 5'- cGUGAGCgAGCGGUGgaaUGCCcgcAGAugcucgcCCGAc -3' miRNA: 3'- cCACUCGgUUGCCAC---AUGG---UCU-------GGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 32176 | 0.67 | 0.807372 |
Target: 5'- cGGUGAGaCCcACGGuUGUGCaCGuGCUGAc -3' miRNA: 3'- -CCACUC-GGuUGCC-ACAUG-GUcUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 37648 | 0.67 | 0.807372 |
Target: 5'- cGGUGuGaCCGACGaUGuUACCgAGACCGu -3' miRNA: 3'- -CCACuC-GGUUGCcAC-AUGG-UCUGGCu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 9719 | 0.67 | 0.778599 |
Target: 5'- --aGA-CCAGCGGUGUG-CAGACCu- -3' miRNA: 3'- ccaCUcGGUUGCCACAUgGUCUGGcu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 27523 | 0.67 | 0.772676 |
Target: 5'- cGUGAcGCCGucgagcacgaccucgGCGGUGgcgGgCAGACCGu -3' miRNA: 3'- cCACU-CGGU---------------UGCCACa--UgGUCUGGCu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 43063 | 0.67 | 0.758665 |
Target: 5'- cGGUGAGCgCAcgcCGGUGc-CCGGcccGCCGAc -3' miRNA: 3'- -CCACUCG-GUu--GCCACauGGUC---UGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 51184 | 0.68 | 0.716329 |
Target: 5'- cGGUGAGCuCAGCgaggccaucgacgGGUGgGCCgAGGCCa- -3' miRNA: 3'- -CCACUCG-GUUG-------------CCACaUGG-UCUGGcu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 7985 | 0.68 | 0.706828 |
Target: 5'- cGGUGAGCUuccacuCGGUGgcgGCgAggucGACCGAc -3' miRNA: 3'- -CCACUCGGuu----GCCACa--UGgU----CUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 16440 | 0.68 | 0.706828 |
Target: 5'- --cGAGCCcgacAUGGUcgACCGGACCGGc -3' miRNA: 3'- ccaCUCGGu---UGCCAcaUGGUCUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 26508 | 0.69 | 0.696205 |
Target: 5'- --aGAGCCGugacccguccgGCGGUGUgGCCAGcaGCCGu -3' miRNA: 3'- ccaCUCGGU-----------UGCCACA-UGGUC--UGGCu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 1851 | 0.69 | 0.673714 |
Target: 5'- uGUGAGCCGcuugucgAUGGUGgcgGCCAugccGCCGAu -3' miRNA: 3'- cCACUCGGU-------UGCCACa--UGGUc---UGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 31331 | 0.69 | 0.664018 |
Target: 5'- cGGUGccGCCAcgguUGGUGUaugugcccgcGCCGGACUGAu -3' miRNA: 3'- -CCACu-CGGUu---GCCACA----------UGGUCUGGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 42189 | 0.7 | 0.631569 |
Target: 5'- cGGUuGGCCGguaccgGCGGUgGUGCCGGGgCGGu -3' miRNA: 3'- -CCAcUCGGU------UGCCA-CAUGGUCUgGCU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 28557 | 0.7 | 0.599124 |
Target: 5'- uGGUGuguGGCCugcCGGUGUgguugacguacuGCCGGGCCGc -3' miRNA: 3'- -CCAC---UCGGuu-GCCACA------------UGGUCUGGCu -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 29993 | 0.7 | 0.599124 |
Target: 5'- -uUGAGC--GCGGUG-ACCAGACCcGAg -3' miRNA: 3'- ccACUCGguUGCCACaUGGUCUGG-CU- -5' |
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23190 | 3' | -54.3 | NC_005259.1 | + | 7405 | 0.72 | 0.514364 |
Target: 5'- cGGUGGGCCggUGGgGUcggcggggaacAgCAGACCGGc -3' miRNA: 3'- -CCACUCGGuuGCCaCA-----------UgGUCUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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