miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23192 5' -59.4 NC_005259.1 + 4491 1.07 0.000655
Target:  5'- uAUCCGCACGGCGUGCGGUCAGUCGUCa -3'
miRNA:   3'- -UAGGCGUGCCGCACGCCAGUCAGCAG- -5'
23192 5' -59.4 NC_005259.1 + 49045 0.72 0.230331
Target:  5'- cGUCCGCAaCGGCGgcucgGCGGUguGggugccCGUCu -3'
miRNA:   3'- -UAGGCGU-GCCGCa----CGCCAguCa-----GCAG- -5'
23192 5' -59.4 NC_005259.1 + 48940 0.7 0.324175
Target:  5'- uGUCCGCuGCGGUG-GCGGUCGucaccaCGUCg -3'
miRNA:   3'- -UAGGCG-UGCCGCaCGCCAGUca----GCAG- -5'
23192 5' -59.4 NC_005259.1 + 48312 0.67 0.482134
Target:  5'- -gUCGCACGGCacGCGcUCGGUgGUCg -3'
miRNA:   3'- uaGGCGUGCCGcaCGCcAGUCAgCAG- -5'
23192 5' -59.4 NC_005259.1 + 28434 0.67 0.511994
Target:  5'- -cCCGCGCGGUGuUGCuGUCGG-CGg- -3'
miRNA:   3'- uaGGCGUGCCGC-ACGcCAGUCaGCag -5'
23192 5' -59.4 NC_005259.1 + 59434 0.66 0.53232
Target:  5'- -aCCGCGuCGGCGaggUGCucGUCAacGUCGUCg -3'
miRNA:   3'- uaGGCGU-GCCGC---ACGc-CAGU--CAGCAG- -5'
23192 5' -59.4 NC_005259.1 + 7492 0.66 0.542592
Target:  5'- --gCGCuCGGUGUGcCGGUCGaccUCGUCg -3'
miRNA:   3'- uagGCGuGCCGCAC-GCCAGUc--AGCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.