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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23192 | 5' | -59.4 | NC_005259.1 | + | 4491 | 1.07 | 0.000655 |
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Target: 5'- uAUCCGCACGGCGUGCGGUCAGUCGUCa -3' miRNA: 3'- -UAGGCGUGCCGCACGCCAGUCAGCAG- -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 49045 | 0.72 | 0.230331 |
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Target: 5'- cGUCCGCAaCGGCGgcucgGCGGUguGggugccCGUCu -3' miRNA: 3'- -UAGGCGU-GCCGCa----CGCCAguCa-----GCAG- -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 48940 | 0.7 | 0.324175 |
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Target: 5'- uGUCCGCuGCGGUG-GCGGUCGucaccaCGUCg -3' miRNA: 3'- -UAGGCG-UGCCGCaCGCCAGUca----GCAG- -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 48312 | 0.67 | 0.482134 |
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Target: 5'- -gUCGCACGGCacGCGcUCGGUgGUCg -3' miRNA: 3'- uaGGCGUGCCGcaCGCcAGUCAgCAG- -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 28434 | 0.67 | 0.511994 |
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Target: 5'- -cCCGCGCGGUGuUGCuGUCGG-CGg- -3' miRNA: 3'- uaGGCGUGCCGC-ACGcCAGUCaGCag -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 59434 | 0.66 | 0.53232 |
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Target: 5'- -aCCGCGuCGGCGaggUGCucGUCAacGUCGUCg -3' miRNA: 3'- uaGGCGU-GCCGC---ACGc-CAGU--CAGCAG- -5' |
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| 23192 | 5' | -59.4 | NC_005259.1 | + | 7492 | 0.66 | 0.542592 |
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Target: 5'- --gCGCuCGGUGUGcCGGUCGaccUCGUCg -3' miRNA: 3'- uagGCGuGCCGCAC-GCCAGUc--AGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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