Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23193 | 3' | -55.6 | NC_005259.1 | + | 4898 | 1.04 | 0.002114 |
Target: 5'- cAUGCACUCGACCGCGCGCUUGAUCAAu -3' miRNA: 3'- -UACGUGAGCUGGCGCGCGAACUAGUU- -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 59492 | 0.71 | 0.389413 |
Target: 5'- cGUGCGCUCG-UCGCGCGCgucgaccgccgccgGGUCAc -3' miRNA: 3'- -UACGUGAGCuGGCGCGCGaa------------CUAGUu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 5214 | 0.69 | 0.527467 |
Target: 5'- cGUG-ACUUGGCCGCGUuCUUGAUCu- -3' miRNA: 3'- -UACgUGAGCUGGCGCGcGAACUAGuu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 46393 | 0.67 | 0.625782 |
Target: 5'- cUGCACgccgcCGACCGC-CGCccGAUCAu -3' miRNA: 3'- uACGUGa----GCUGGCGcGCGaaCUAGUu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 17234 | 0.67 | 0.625782 |
Target: 5'- uUGCGCUCGGCgaGCGC-CUUGAg--- -3' miRNA: 3'- uACGUGAGCUGg-CGCGcGAACUaguu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 15994 | 0.67 | 0.658978 |
Target: 5'- uUGC-CUCGACUGgGCGCUgcggUGGcgUCGAg -3' miRNA: 3'- uACGuGAGCUGGCgCGCGA----ACU--AGUU- -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 62630 | 0.67 | 0.658978 |
Target: 5'- -cGUugUCG-CCGgGCGCgaUGGUCAAc -3' miRNA: 3'- uaCGugAGCuGGCgCGCGa-ACUAGUU- -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 20543 | 0.67 | 0.658978 |
Target: 5'- -aGCGCguUCcACCGCGCGCcgGGUCGc -3' miRNA: 3'- uaCGUG--AGcUGGCGCGCGaaCUAGUu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 36134 | 0.66 | 0.670008 |
Target: 5'- uUGaC-CUCGA-CGCGCGCggUGAUCGAu -3' miRNA: 3'- uAC-GuGAGCUgGCGCGCGa-ACUAGUU- -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 33676 | 0.66 | 0.681001 |
Target: 5'- uGUGUACgCGACCcCGCGUagcgcgUGAUCGAg -3' miRNA: 3'- -UACGUGaGCUGGcGCGCGa-----ACUAGUU- -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 7146 | 0.66 | 0.702835 |
Target: 5'- -aGCAcCUCGGCCaccgccucGCGUGCcgcgUGAUCAc -3' miRNA: 3'- uaCGU-GAGCUGG--------CGCGCGa---ACUAGUu -5' |
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23193 | 3' | -55.6 | NC_005259.1 | + | 24260 | 0.66 | 0.72439 |
Target: 5'- -cGCcCUCGACCGCGCcgagGCUcuUGAgCAc -3' miRNA: 3'- uaCGuGAGCUGGCGCG----CGA--ACUaGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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