Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23194 | 3' | -54.1 | NC_005259.1 | + | 4367 | 0.66 | 0.788009 |
Target: 5'- --cGGcacgcccgUGCCC-UGCUCAUcGCCaAGCa -3' miRNA: 3'- uuaCCa-------ACGGGaACGAGUA-CGGcUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 67112 | 0.66 | 0.757374 |
Target: 5'- --aGGUguacUGCCCgcUGCggUCGUGCgUGAGCa -3' miRNA: 3'- uuaCCA----ACGGGa-ACG--AGUACG-GCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 26828 | 0.67 | 0.724477 |
Target: 5'- cGGUGG-UGCCggggcggucUGCUCGggcaggaUGCCGAGCu -3' miRNA: 3'- -UUACCaACGGga-------ACGAGU-------ACGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 7309 | 0.67 | 0.714741 |
Target: 5'- -uUGGgUGCCCgaGUUCAccgccgucGCCGAGUa -3' miRNA: 3'- uuACCaACGGGaaCGAGUa-------CGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 5692 | 0.67 | 0.714741 |
Target: 5'- -----cUGCCUgcugUGCUCGUGCCGccaaGGCc -3' miRNA: 3'- uuaccaACGGGa---ACGAGUACGGC----UCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 26055 | 0.67 | 0.703843 |
Target: 5'- --gGGUUGgCCaUGC-CGccGCCGAGCa -3' miRNA: 3'- uuaCCAACgGGaACGaGUa-CGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 55105 | 0.68 | 0.659648 |
Target: 5'- cGAUGGcggcgaaaaaUGCaCCggcUGC-CGUGCCGAGCa -3' miRNA: 3'- -UUACCa---------ACG-GGa--ACGaGUACGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 66807 | 0.7 | 0.567343 |
Target: 5'- cGAUGGUgcgUGCCCUgccggucucgaaacUcugGCUCAUGCCGcccGCa -3' miRNA: 3'- -UUACCA---ACGGGA--------------A---CGAGUACGGCu--CG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 56406 | 0.7 | 0.548729 |
Target: 5'- cGAUGGUgcccgGCCCgccgaGCUCAaGCaGAGCa -3' miRNA: 3'- -UUACCAa----CGGGaa---CGAGUaCGgCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 43839 | 0.7 | 0.527082 |
Target: 5'- --aGGgccGCCgCUcGC-CAUGCCGAGCg -3' miRNA: 3'- uuaCCaa-CGG-GAaCGaGUACGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 22917 | 0.7 | 0.527082 |
Target: 5'- --aGGaUGCCCUcgUGCUCGaccucGCCGGGUc -3' miRNA: 3'- uuaCCaACGGGA--ACGAGUa----CGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 36203 | 0.73 | 0.40565 |
Target: 5'- -----aUGCCCaUGCUCGacagcgcgaUGCCGAGCa -3' miRNA: 3'- uuaccaACGGGaACGAGU---------ACGGCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 44304 | 0.73 | 0.387159 |
Target: 5'- --gGGUUGCCCUcuuUGaCgagCGUGuuGAGCg -3' miRNA: 3'- uuaCCAACGGGA---AC-Ga--GUACggCUCG- -5' |
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23194 | 3' | -54.1 | NC_005259.1 | + | 63408 | 1.1 | 0.001066 |
Target: 5'- cAAUGGUUGCCCUUGCUCAUGCCGAGCa -3' miRNA: 3'- -UUACCAACGGGAACGAGUACGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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