Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23195 | 3' | -56.8 | NC_005259.1 | + | 36236 | 0.7 | 0.448571 |
Target: 5'- -uUGGCCAGCGCgaGCGccUGCGUcGGCa -3' miRNA: 3'- gcACCGGUCGUGg-CGUa-ACGCA-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 22208 | 0.69 | 0.488187 |
Target: 5'- aCGuUGGCCAccgcGCACCGCc--GCGaGGCUg -3' miRNA: 3'- -GC-ACCGGU----CGUGGCGuaaCGCaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 41663 | 0.69 | 0.498349 |
Target: 5'- gGUGaGCgGgaucuGCACCGCGU-GCGUGGCc -3' miRNA: 3'- gCAC-CGgU-----CGUGGCGUAaCGCAUCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 23596 | 0.68 | 0.550445 |
Target: 5'- gCGUGGCaCGGCAguuccCCGCGUgGCGgcAGCc -3' miRNA: 3'- -GCACCG-GUCGU-----GGCGUAaCGCa-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 8770 | 0.68 | 0.561078 |
Target: 5'- -uUGGCCAGCGCgGCggUGaugAGCUc -3' miRNA: 3'- gcACCGGUCGUGgCGuaACgcaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 38746 | 0.66 | 0.690627 |
Target: 5'- gGUGGCC--CGCCGCAggccgGUG-AGCUg -3' miRNA: 3'- gCACCGGucGUGGCGUaa---CGCaUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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