Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23195 | 5' | -59.3 | NC_005259.1 | + | 63912 | 0.66 | 0.619048 |
Target: 5'- gCGAGuCGAGGUCGCGAUugaaCUCGUgggUCUUa -3' miRNA: 3'- -GCUC-GCUCCGGUGCUG----GAGCA---GGAGc -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 8367 | 0.66 | 0.614883 |
Target: 5'- --cGCucGGCgGCGGCCUCGgcaucggcgagagCCUCGg -3' miRNA: 3'- gcuCGcuCCGgUGCUGGAGCa------------GGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 43960 | 0.66 | 0.608639 |
Target: 5'- uGGGCGGGGCCGCcGCCU-GaCCgagggCGg -3' miRNA: 3'- gCUCGCUCCGGUGcUGGAgCaGGa----GC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 4225 | 0.66 | 0.598248 |
Target: 5'- uCGAGC-AGGCCAagcaGGCCccgcccaCGUgCCUCGa -3' miRNA: 3'- -GCUCGcUCCGGUg---CUGGa------GCA-GGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 42474 | 0.66 | 0.578583 |
Target: 5'- -cGGCGAGGCC-CGGCCcguagagccgugaccCGUCCggCGg -3' miRNA: 3'- gcUCGCUCCGGuGCUGGa--------------GCAGGa-GC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 23958 | 0.67 | 0.546826 |
Target: 5'- cCGAGaCG-GGCCGauUGACCUCGUaCC-CGc -3' miRNA: 3'- -GCUC-GCuCCGGU--GCUGGAGCA-GGaGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 11304 | 0.67 | 0.516638 |
Target: 5'- aCGGGCguGAGGCCGaGACgggcgcgcagCUCGUCgUCGg -3' miRNA: 3'- -GCUCG--CUCCGGUgCUG----------GAGCAGgAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 60197 | 0.68 | 0.496889 |
Target: 5'- cCGAcCG-GGCCACGAUCUCGccgaUCUCGu -3' miRNA: 3'- -GCUcGCuCCGGUGCUGGAGCa---GGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 18878 | 0.69 | 0.439856 |
Target: 5'- gCGuGgGAGGCCaaccACGAUgUCGaCCUCGg -3' miRNA: 3'- -GCuCgCUCCGG----UGCUGgAGCaGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 3830 | 0.69 | 0.439856 |
Target: 5'- ---aCGGGGCCACGACgaUGUcCCUCGc -3' miRNA: 3'- gcucGCUCCGGUGCUGgaGCA-GGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 51436 | 0.7 | 0.369997 |
Target: 5'- uGGGCG-GGCCACGggGCCUCGcgCCg-- -3' miRNA: 3'- gCUCGCuCCGGUGC--UGGAGCa-GGagc -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 63573 | 0.71 | 0.330356 |
Target: 5'- aCGAGCGGGauauacgccGCCugGcuguUCUCGUCCUCc -3' miRNA: 3'- -GCUCGCUC---------CGGugCu---GGAGCAGGAGc -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 62896 | 0.71 | 0.315399 |
Target: 5'- uGAGCGAcGCCGCuuCCucgguacuacgcUCGUCCUCGg -3' miRNA: 3'- gCUCGCUcCGGUGcuGG------------AGCAGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 33360 | 0.71 | 0.308115 |
Target: 5'- uCGGGCGAGGUCAUGACuCUCcuaccgGUCCg-- -3' miRNA: 3'- -GCUCGCUCCGGUGCUG-GAG------CAGGagc -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 50328 | 0.73 | 0.254455 |
Target: 5'- aCGAGCuc-GUCGCGGcCCUUGUCCUCGg -3' miRNA: 3'- -GCUCGcucCGGUGCU-GGAGCAGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 67810 | 0.74 | 0.20352 |
Target: 5'- gCGAGCuuGaGCC-CGAUCUUGUCCUCGa -3' miRNA: 3'- -GCUCGcuC-CGGuGCUGGAGCAGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 62564 | 0.74 | 0.197939 |
Target: 5'- cCGGGCaGcGGCCacaucagACGACCUCGcCCUCGa -3' miRNA: 3'- -GCUCG-CuCCGG-------UGCUGGAGCaGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 46478 | 0.75 | 0.193473 |
Target: 5'- uGAGCGcuguGGCCACGcCCUugaucgUGUCCUCGu -3' miRNA: 3'- gCUCGCu---CCGGUGCuGGA------GCAGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 49588 | 0.78 | 0.114862 |
Target: 5'- gCGGGCGGGGUCAgcACCUCGaCCUCGg -3' miRNA: 3'- -GCUCGCUCCGGUgcUGGAGCaGGAGC- -5' |
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23195 | 5' | -59.3 | NC_005259.1 | + | 62668 | 1.09 | 0.000664 |
Target: 5'- cCGAGCGAGGCCACGACCUCGUCCUCGa -3' miRNA: 3'- -GCUCGCUCCGGUGCUGGAGCAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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