Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23197 | 3' | -57.8 | NC_005259.1 | + | 35603 | 0.66 | 0.655717 |
Target: 5'- gGGUgCGUCcUGGCCACgcugcgucgaguagUUCGGGCCGCc -3' miRNA: 3'- -CUA-GCAGaGCUGGUG--------------GAGUCCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 58077 | 0.66 | 0.648254 |
Target: 5'- -----aUUCGACCAgCUCGGGCCaGCc -3' miRNA: 3'- cuagcaGAGCUGGUgGAGUCCGG-CGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 49536 | 0.66 | 0.626903 |
Target: 5'- -cUCGaacgUGGCCACCUCAGuGCCGg- -3' miRNA: 3'- cuAGCaga-GCUGGUGGAGUC-CGGCgu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 45005 | 0.66 | 0.61623 |
Target: 5'- --cCG-C-CGACCGCCg-GGGCCGCGc -3' miRNA: 3'- cuaGCaGaGCUGGUGGagUCCGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 56397 | 0.66 | 0.60557 |
Target: 5'- uGAUCGUCUCGAUggUGCC-CGGcCCGCc -3' miRNA: 3'- -CUAGCAGAGCUG--GUGGaGUCcGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 2477 | 0.67 | 0.584323 |
Target: 5'- -uUCGUCgc--UCGCCUCAauGGCCGCAc -3' miRNA: 3'- cuAGCAGagcuGGUGGAGU--CCGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 24465 | 0.67 | 0.552751 |
Target: 5'- aGAUCGUCUCGACCGgagagcucguCCUC-GGCg--- -3' miRNA: 3'- -CUAGCAGAGCUGGU----------GGAGuCCGgcgu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 62072 | 0.67 | 0.542336 |
Target: 5'- gGGUCGgcauagacacgCUCGACCGCCUCAacgaucucGUCGCGc -3' miRNA: 3'- -CUAGCa----------GAGCUGGUGGAGUc-------CGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 55021 | 0.67 | 0.542336 |
Target: 5'- cGGUCGUCUCGGCa----CGGGCUGCc -3' miRNA: 3'- -CUAGCAGAGCUGguggaGUCCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 39973 | 0.68 | 0.531987 |
Target: 5'- --gCGacugCUCGACCACCUCgacGGGCUGa- -3' miRNA: 3'- cuaGCa---GAGCUGGUGGAG---UCCGGCgu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 48477 | 0.68 | 0.531987 |
Target: 5'- gGGUCGcgUCGGCCuguuCCUCgacgcgcuugaGGGCCGCGa -3' miRNA: 3'- -CUAGCagAGCUGGu---GGAG-----------UCCGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 39896 | 0.68 | 0.531987 |
Target: 5'- cGUUGUCgccgcCGcCCGCCUCgauccgucgaGGGCCGCGu -3' miRNA: 3'- cUAGCAGa----GCuGGUGGAG----------UCCGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 58188 | 0.68 | 0.49137 |
Target: 5'- --aCGUCUUGACCGCCgUGGGCUuGCc -3' miRNA: 3'- cuaGCAGAGCUGGUGGaGUCCGG-CGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 19247 | 0.7 | 0.414886 |
Target: 5'- uGAUCGa--UGGCCGCCUgcCGGGCCGCc -3' miRNA: 3'- -CUAGCagaGCUGGUGGA--GUCCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 42242 | 0.7 | 0.405846 |
Target: 5'- ----aUCUCGACCACCUCGcGCUGUg -3' miRNA: 3'- cuagcAGAGCUGGUGGAGUcCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 52000 | 0.71 | 0.362556 |
Target: 5'- --cCGaUCUCGACCggaugcacgaGCgUCAGGCCGCc -3' miRNA: 3'- cuaGC-AGAGCUGG----------UGgAGUCCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 38656 | 0.71 | 0.362556 |
Target: 5'- -cUCGccgaaaUCGACCACCUCGcuguuGGCCGCc -3' miRNA: 3'- cuAGCag----AGCUGGUGGAGU-----CCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 45723 | 0.72 | 0.307533 |
Target: 5'- aGAUCGcCUgCGcACCACCg-AGGCCGCGc -3' miRNA: 3'- -CUAGCaGA-GC-UGGUGGagUCCGGCGU- -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 30145 | 0.73 | 0.259126 |
Target: 5'- -cUCGggcagCUCGACCgggccGCCUaCAGGCCGCc -3' miRNA: 3'- cuAGCa----GAGCUGG-----UGGA-GUCCGGCGu -5' |
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23197 | 3' | -57.8 | NC_005259.1 | + | 8901 | 0.73 | 0.259126 |
Target: 5'- --aCG-CUCGGCCugCUgaCGGGCCGCGu -3' miRNA: 3'- cuaGCaGAGCUGGugGA--GUCCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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