Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23198 | 3' | -49.3 | NC_005259.1 | + | 56553 | 0.66 | 0.983645 |
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Target: 5'- cUCGUCGGUcucgGGUucg--GUGAucGUCGCGg -3' miRNA: 3'- -GGUAGCCG----CCGuuuuaCACU--UAGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 57017 | 0.66 | 0.973777 |
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Target: 5'- cUCAgcgCGGCucGGCGcgGUG-GggUCGCa -3' miRNA: 3'- -GGUa--GCCG--CCGUuuUACaCuuAGCGc -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 58446 | 0.71 | 0.844669 |
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Target: 5'- -gAUCGGUGGCcuug-GUG-AUCGCGg -3' miRNA: 3'- ggUAGCCGCCGuuuuaCACuUAGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 60603 | 0.81 | 0.332654 |
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Target: 5'- gCCGUCaucgagcagcacaGGCGGCGAGGUGUGGAacugCGCGu -3' miRNA: 3'- -GGUAG-------------CCGCCGUUUUACACUUa---GCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 60963 | 0.66 | 0.98127 |
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Target: 5'- gCGUcCGGCGGCAGAucgaaccAUuccucuucgGUGAGcUCGCGc -3' miRNA: 3'- gGUA-GCCGCCGUUU-------UA---------CACUU-AGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 61673 | 1.13 | 0.003281 |
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Target: 5'- cCCAUCGGCGGCAAAAUGUGAAUCGCGg -3' miRNA: 3'- -GGUAGCCGCCGUUUUACACUUAGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 62738 | 0.71 | 0.853368 |
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Target: 5'- aCCGgacCGGCGGCAGuacGcgGUGGuacUCGUGg -3' miRNA: 3'- -GGUa--GCCGCCGUU---UuaCACUu--AGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 64813 | 0.66 | 0.981496 |
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Target: 5'- gCC-UCGGCGGCAAugagcGUGUGG--UGaCGg -3' miRNA: 3'- -GGuAGCCGCCGUUu----UACACUuaGC-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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