Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23198 | 5' | -54.1 | NC_005259.1 | + | 50395 | 0.65 | 0.847088 |
Target: 5'- uGUCGCGGcUCGCcgagcgcgccgguGCCUUGaccggcUCGGGCAGc -3' miRNA: 3'- -CAGCGCUaGGUG-------------UGGAAC------AGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 10733 | 0.66 | 0.815878 |
Target: 5'- uGUCgGUGAUcaCCGCGCgCUgccgggcgaucaucaUGUCGGGCGGg -3' miRNA: 3'- -CAG-CGCUA--GGUGUG-GA---------------ACAGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 49572 | 0.66 | 0.802716 |
Target: 5'- gGUCGUGG-CCAgACCgg--CGGGCGGg -3' miRNA: 3'- -CAGCGCUaGGUgUGGaacaGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 7227 | 0.66 | 0.802716 |
Target: 5'- -gCGUGAUgaacgacggcagCCGCGCCggUGUCGAGUg- -3' miRNA: 3'- caGCGCUA------------GGUGUGGa-ACAGCUCGuc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 37826 | 0.67 | 0.783321 |
Target: 5'- cUCGaccuCGggCCACACCUgcGUCGAGCc- -3' miRNA: 3'- cAGC----GCuaGGUGUGGAa-CAGCUCGuc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 3998 | 0.67 | 0.783321 |
Target: 5'- aUgGCGggCCGCGuCCcccgGUUGAGCAGa -3' miRNA: 3'- cAgCGCuaGGUGU-GGaa--CAGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 33588 | 0.67 | 0.783321 |
Target: 5'- cGUCGCGggCCACcgagGCgcgGUCGAGCu- -3' miRNA: 3'- -CAGCGCuaGGUG----UGgaaCAGCUCGuc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 30794 | 0.67 | 0.783321 |
Target: 5'- uUCGCGuaccgCCggaACACCUgaGUCGGGUAGu -3' miRNA: 3'- cAGCGCua---GG---UGUGGAa-CAGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 43713 | 0.67 | 0.763304 |
Target: 5'- -gCGCGA-CCGCAUCgaGUCGAugGCGGc -3' miRNA: 3'- caGCGCUaGGUGUGGaaCAGCU--CGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 51618 | 0.67 | 0.753091 |
Target: 5'- cUCGcCGGUCCACACgagGUCgucgGAGCGGu -3' miRNA: 3'- cAGC-GCUAGGUGUGgaaCAG----CUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 17749 | 0.67 | 0.752062 |
Target: 5'- -aCGCGAUCCgugaggccgccgcGCGCCggGcCGAGCGc -3' miRNA: 3'- caGCGCUAGG-------------UGUGGaaCaGCUCGUc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 1938 | 0.68 | 0.732309 |
Target: 5'- -cCGaCGGUCCACACCggcgUGAGCGu -3' miRNA: 3'- caGC-GCUAGGUGUGGaacaGCUCGUc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 35605 | 0.68 | 0.721763 |
Target: 5'- -gUGCGuccuggCCACGCUgcGUCGAGUAGu -3' miRNA: 3'- caGCGCua----GGUGUGGaaCAGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 46572 | 0.69 | 0.667938 |
Target: 5'- gGUCGCGGcgucgaCCGCGCCgcccgaggCGAGCAGc -3' miRNA: 3'- -CAGCGCUa-----GGUGUGGaaca----GCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 36219 | 0.69 | 0.6461 |
Target: 5'- -gCGCGAUgCCgaGCACCUUGgccagcgCGAGCGc -3' miRNA: 3'- caGCGCUA-GG--UGUGGAACa------GCUCGUc -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 1579 | 0.69 | 0.635157 |
Target: 5'- -gCGCGccCCACACCgagGUCGAGguCAGg -3' miRNA: 3'- caGCGCuaGGUGUGGaa-CAGCUC--GUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 16685 | 0.71 | 0.527059 |
Target: 5'- gGUCgGCGAUCCuggGCACCgagGUgaccgcCGAGCAGg -3' miRNA: 3'- -CAG-CGCUAGG---UGUGGaa-CA------GCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 66112 | 0.72 | 0.50615 |
Target: 5'- -gCGCGAcuUCUACGCgc-GUCGAGCAGg -3' miRNA: 3'- caGCGCU--AGGUGUGgaaCAGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 26129 | 0.72 | 0.47548 |
Target: 5'- gGUCGcCGGUCCgACGCCgccgagGcCGAGCAGc -3' miRNA: 3'- -CAGC-GCUAGG-UGUGGaa----CaGCUCGUC- -5' |
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23198 | 5' | -54.1 | NC_005259.1 | + | 9417 | 0.79 | 0.183059 |
Target: 5'- --gGUGcgCCACACCgUUGUCGAGCAGc -3' miRNA: 3'- cagCGCuaGGUGUGG-AACAGCUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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