Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
232 | 3' | -51.1 | AC_000008.1 | + | 9575 | 0.66 | 0.863166 |
Target: 5'- gGGGGCUGccAUGCG--GCAgggaUACGGc -3' miRNA: 3'- -CCCCGACuuUGCGCuuUGUag--GUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 17514 | 0.66 | 0.85363 |
Target: 5'- cGGGGCgcGGAcgcuuuucgccACGCcccucugGAGACAcugucUCCACGGc -3' miRNA: 3'- -CCCCGa-CUU-----------UGCG-------CUUUGU-----AGGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 12189 | 0.66 | 0.850976 |
Target: 5'- uGGGCUGGcgguggcacccgcGCGCGcuggcAACGUCgGCGGc -3' miRNA: 3'- cCCCGACUu------------UGCGCu----UUGUAGgUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 6696 | 0.66 | 0.836441 |
Target: 5'- uGGGGUggguGAGCGCGGAggcguACAUgCCGCa- -3' miRNA: 3'- -CCCCGac--UUUGCGCUU-----UGUA-GGUGcc -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 23899 | 0.66 | 0.836441 |
Target: 5'- cGGcGGCgacgGGGACGgGGacGACAcguccUCCAUGGu -3' miRNA: 3'- -CC-CCGa---CUUUGCgCU--UUGU-----AGGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 32085 | 0.67 | 0.817432 |
Target: 5'- cGGuGGCUGcAGCGgcUGAAGCggCgGCGGa -3' miRNA: 3'- -CC-CCGACuUUGC--GCUUUGuaGgUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 549 | 0.67 | 0.807601 |
Target: 5'- cGGGaCUGAAAa-UGAGACAUauuaucugCCACGGa -3' miRNA: 3'- cCCC-GACUUUgcGCUUUGUA--------GGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 6019 | 0.67 | 0.797569 |
Target: 5'- gGGGGUgGggGCGCGuu-CGUCCuCa- -3' miRNA: 3'- -CCCCGaCuuUGCGCuuuGUAGGuGcc -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 18363 | 0.67 | 0.776948 |
Target: 5'- cGGGCUGGcGACgGCGAcGGCggCgGCGGg -3' miRNA: 3'- cCCCGACU-UUG-CGCU-UUGuaGgUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 17002 | 0.68 | 0.766384 |
Target: 5'- cGGGGCUcaGguACucCGAGGCGUCCugGc -3' miRNA: 3'- -CCCCGA--CuuUGc-GCUUUGUAGGugCc -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 7668 | 0.68 | 0.75567 |
Target: 5'- aGGGUUgGAAGCGaCGAAugAgcUCCACaGGu -3' miRNA: 3'- cCCCGA-CUUUGC-GCUUugU--AGGUG-CC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 20231 | 0.68 | 0.744817 |
Target: 5'- gGGGGCUGGcgGGCGCGccaAAAUcugGUUCuCGGg -3' miRNA: 3'- -CCCCGACU--UUGCGC---UUUG---UAGGuGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 24544 | 0.68 | 0.744817 |
Target: 5'- cGGGCUccucucccucgcGGu-CGCGAAACAgguucugCCGCGGc -3' miRNA: 3'- cCCCGA------------CUuuGCGCUUUGUa------GGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 10900 | 0.68 | 0.744817 |
Target: 5'- aGGGUUGAGuCGCGGGACc-CC-CGGu -3' miRNA: 3'- cCCCGACUUuGCGCUUUGuaGGuGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 12558 | 0.7 | 0.631557 |
Target: 5'- -uGGCUG-AACGaGGAGCA-CCGCGGg -3' miRNA: 3'- ccCCGACuUUGCgCUUUGUaGGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 49 | 0.71 | 0.597037 |
Target: 5'- aGGGGgUGGAguuugugacgugGCGCGggGCGUgggaACGGg -3' miRNA: 3'- -CCCCgACUU------------UGCGCuuUGUAgg--UGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 49 | 0.71 | 0.597037 |
Target: 5'- aGGGGgUGGAguuugugacgugGCGCGggGCGUgggaACGGg -3' miRNA: 3'- -CCCCgACUU------------UGCGCuuUGUAgg--UGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 11279 | 0.72 | 0.533581 |
Target: 5'- aGGGugcaGCUGAAGCGUGAuacgcgugaggcguACGUgCCGCGGc -3' miRNA: 3'- -CCC----CGACUUUGCGCUu-------------UGUA-GGUGCC- -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 17105 | 0.73 | 0.474803 |
Target: 5'- uGGGCUGGAGCcCGAG--GUCCGCGu -3' miRNA: 3'- cCCCGACUUUGcGCUUugUAGGUGCc -5' |
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232 | 3' | -51.1 | AC_000008.1 | + | 8463 | 0.73 | 0.443573 |
Target: 5'- aGGGGCUGGuugGugGCG--GCGUCgAUGGc -3' miRNA: 3'- -CCCCGACU---UugCGCuuUGUAGgUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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