miRNA: 3'- -CGGA-GUUUCU----------UCCGGuaa--CCCGGGCC- -5'
miRNA: 3'- -CGGAGuuuCuuCCGGuaAC----CCGGGCC- -5'
miRNA: 3'- -CGGAGUUUCUUCCGGUAACCCGGGCC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2320 | 3' | -54.9 | NC_001414.1 | + | 2188 | 0.71 | 0.087609 |
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Target: 5'- uGCCUcCAGAGAgcgauggaccAGGUCuugaaGGGCCUGGa -3' miRNA: 3'- -CGGA-GUUUCU----------UCCGGuaa--CCCGGGCC- -5' |
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| 2320 | 3' | -54.9 | NC_001414.1 | + | 1451 | 0.76 | 0.036653 |
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Target: 5'- gGCCUUuu-GggGGCCggUGccgaGGCCCGGg -3' miRNA: 3'- -CGGAGuuuCuuCCGGuaAC----CCGGGCC- -5' |
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| 2320 | 3' | -54.9 | NC_001414.1 | + | 1467 | 1.13 | 3.2e-05 |
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Target: 5'- uGCCUCAAAGAAGGCCAUUGGGCCCGGg -3' miRNA: 3'- -CGGAGUUUCUUCCGGUAACCCGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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