Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23201 | 3' | -58.6 | NC_005259.1 | + | 62764 | 0.66 | 0.622725 |
Target: 5'- uACUCGuGGGCCGGAA--CGUCGcGCa-- -3' miRNA: 3'- -UGGGC-UCCGGCCUUuaGCAGC-CGcua -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 47030 | 0.66 | 0.612123 |
Target: 5'- cGCCUccaGcAGGCCGGGGAUCugcgcgagcguGUUGGUGAg -3' miRNA: 3'- -UGGG---C-UCCGGCCUUUAG-----------CAGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 31684 | 0.66 | 0.612123 |
Target: 5'- uGCCCGAGGC-----AUCcUCGGCGGUg -3' miRNA: 3'- -UGGGCUCCGgccuuUAGcAGCCGCUA- -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 38278 | 0.66 | 0.601537 |
Target: 5'- cCCCGAGGUgagaaugucauCGGcgcuGAUCGUCGGacCGAUc -3' miRNA: 3'- uGGGCUCCG-----------GCCu---UUAGCAGCC--GCUA- -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 39908 | 0.66 | 0.598365 |
Target: 5'- cGCCCGccucgauccgucgaGGGCCGcgucGAGGUCGUCcuuGGUGAc -3' miRNA: 3'- -UGGGC--------------UCCGGC----CUUUAGCAG---CCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 16814 | 0.66 | 0.580446 |
Target: 5'- cGCUCGGGGCCGGGgucauGAUCcgacacaaGGCGGg -3' miRNA: 3'- -UGGGCUCCGGCCU-----UUAGcag-----CCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 45624 | 0.66 | 0.569956 |
Target: 5'- uGCCCGccGGGCCGGGcaGcgCGcCGGUGc- -3' miRNA: 3'- -UGGGC--UCCGGCCU--UuaGCaGCCGCua -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 60532 | 0.67 | 0.549125 |
Target: 5'- -gCCG-GGCCGGGAcgcagccgcUCGUCGGCa-- -3' miRNA: 3'- ugGGCuCCGGCCUUu--------AGCAGCCGcua -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 26807 | 0.67 | 0.528537 |
Target: 5'- cGCCCGGuuGGCCGGuac-CGgCGGUGGUg -3' miRNA: 3'- -UGGGCU--CCGGCCuuuaGCaGCCGCUA- -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 47929 | 0.67 | 0.508241 |
Target: 5'- -gCCGAGGCCcuGGAAGcCGaugaCGGCGGUg -3' miRNA: 3'- ugGGCUCCGG--CCUUUaGCa---GCCGCUA- -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 24019 | 0.68 | 0.492244 |
Target: 5'- cACCaCGGGGCUGGcgaAAuggaaccgccgcaacGUCGUCGGCGc- -3' miRNA: 3'- -UGG-GCUCCGGCC---UU---------------UAGCAGCCGCua -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 64021 | 0.68 | 0.488282 |
Target: 5'- cGCCCGAGGCgCGGcGAg-GcCGGUGAc -3' miRNA: 3'- -UGGGCUCCG-GCCuUUagCaGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 58912 | 0.68 | 0.459059 |
Target: 5'- gGCUCGucGgCGGucuuGUCGUCGGCGGg -3' miRNA: 3'- -UGGGCucCgGCCuu--UAGCAGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 43513 | 0.69 | 0.421601 |
Target: 5'- cCUCGGaacuGCCGGAAAUCGUCGGUc-- -3' miRNA: 3'- uGGGCUc---CGGCCUUUAGCAGCCGcua -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 14022 | 0.69 | 0.403575 |
Target: 5'- uGCCCGAcGGCacgaucaucgagCGGcAGcgcAUCGUCGGCGAc -3' miRNA: 3'- -UGGGCU-CCG------------GCC-UU---UAGCAGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 58962 | 0.7 | 0.37747 |
Target: 5'- gGCCagcAGGUCGGGcagcUCGUCGGCGAc -3' miRNA: 3'- -UGGgc-UCCGGCCUuu--AGCAGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 46592 | 0.71 | 0.313602 |
Target: 5'- cGCCCGAGGC--GAGcagcgcGUCGUUGGCGGUc -3' miRNA: 3'- -UGGGCUCCGgcCUU------UAGCAGCCGCUA- -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 38749 | 0.72 | 0.284872 |
Target: 5'- gGCCCGccgcAGGCCGGugagcugcgcGAUCauGUCGGCGAa -3' miRNA: 3'- -UGGGC----UCCGGCCu---------UUAG--CAGCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 18323 | 0.72 | 0.284872 |
Target: 5'- gACaCCGAGGCCGGugcguUCGUggucgacgccgaCGGCGAc -3' miRNA: 3'- -UG-GGCUCCGGCCuuu--AGCA------------GCCGCUa -5' |
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23201 | 3' | -58.6 | NC_005259.1 | + | 18732 | 0.72 | 0.271305 |
Target: 5'- cGCCCGGcaUCGGuAAGUCGUCGGUGGUg -3' miRNA: 3'- -UGGGCUccGGCC-UUUAGCAGCCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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