Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23201 | 5' | -56.8 | NC_005259.1 | + | 14843 | 0.66 | 0.779295 |
Target: 5'- gCAUCGUGGacacGGUCGU---GCUCGCGa -3' miRNA: 3'- gGUAGCGCU----CCAGCGcucUGAGCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 33815 | 0.66 | 0.778345 |
Target: 5'- aUCAUCGUGAcggGGUCcgGCGAGAgcuuuauCUCGCc- -3' miRNA: 3'- -GGUAGCGCU---CCAG--CGCUCU-------GAGCGcc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 55892 | 0.66 | 0.750239 |
Target: 5'- aCC-UCG-GGGGUCGCGccGGGgUCGCuGGc -3' miRNA: 3'- -GGuAGCgCUCCAGCGC--UCUgAGCG-CC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 41880 | 0.66 | 0.750239 |
Target: 5'- aCCGUgGCGGGccgauacgcaUGCGGGAUgagCGCGGg -3' miRNA: 3'- -GGUAgCGCUCca--------GCGCUCUGa--GCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 29942 | 0.66 | 0.729302 |
Target: 5'- gCCA-CGCGGcccGGUCGCGgcuggugGGGCggGCGGu -3' miRNA: 3'- -GGUaGCGCU---CCAGCGC-------UCUGagCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 32510 | 0.67 | 0.710023 |
Target: 5'- aCAcCGUGAGGUaGuCGAGGCggGCGGc -3' miRNA: 3'- gGUaGCGCUCCAgC-GCUCUGagCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 5335 | 0.67 | 0.710023 |
Target: 5'- gCCGUUGU--GGUgGUGAGAUUCGCa- -3' miRNA: 3'- -GGUAGCGcuCCAgCGCUCUGAGCGcc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 49303 | 0.68 | 0.658264 |
Target: 5'- aCAUCGuCGGGGUccaucaggCGCGAGAauaCGCGu -3' miRNA: 3'- gGUAGC-GCUCCA--------GCGCUCUga-GCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 48433 | 0.68 | 0.658264 |
Target: 5'- aCCA-C-CGAGG-CGCGAGGCa-GCGGc -3' miRNA: 3'- -GGUaGcGCUCCaGCGCUCUGagCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 27837 | 0.68 | 0.62687 |
Target: 5'- ---gCGCGAGGUaggcCGCGAGG-UCGCGc -3' miRNA: 3'- gguaGCGCUCCA----GCGCUCUgAGCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 7640 | 0.68 | 0.625823 |
Target: 5'- aCCGUCGcCGAGuucgaccgccgucGUCGUGAGcgccccgcCUCGUGGg -3' miRNA: 3'- -GGUAGC-GCUC-------------CAGCGCUCu-------GAGCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 53189 | 0.69 | 0.595512 |
Target: 5'- cCCGUCGCGGuGUCcccgGCGAucaGAUUCGCGu -3' miRNA: 3'- -GGUAGCGCUcCAG----CGCU---CUGAGCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 21631 | 0.69 | 0.595512 |
Target: 5'- -uGUCGCGcGGaguguUCGcCGAGGCucUCGCGGa -3' miRNA: 3'- ggUAGCGCuCC-----AGC-GCUCUG--AGCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 58686 | 0.7 | 0.543904 |
Target: 5'- uCCAUCGCGGGGUCG----GCUCGUc- -3' miRNA: 3'- -GGUAGCGCUCCAGCgcucUGAGCGcc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 57965 | 0.7 | 0.523652 |
Target: 5'- cCCAUgGuCGAGGUCGCcGGGA-UCGUGa -3' miRNA: 3'- -GGUAgC-GCUCCAGCG-CUCUgAGCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 28006 | 0.7 | 0.513633 |
Target: 5'- gCCGggGCGGGGUCGCcGGGgUUGCGc -3' miRNA: 3'- -GGUagCGCUCCAGCGcUCUgAGCGCc -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 63897 | 0.71 | 0.455356 |
Target: 5'- uCCAUaucCGCacgagcgagucGAGGUCGCGAuugaACUCGUGGg -3' miRNA: 3'- -GGUA---GCG-----------CUCCAGCGCUc---UGAGCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 57690 | 0.72 | 0.436729 |
Target: 5'- aCCGUgGCcAGGUUGcCGGgcuugcccGACUCGCGGg -3' miRNA: 3'- -GGUAgCGcUCCAGC-GCU--------CUGAGCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 27897 | 0.72 | 0.392161 |
Target: 5'- uCCA-CcUGGGGUCGcCGAGACcgCGCGGg -3' miRNA: 3'- -GGUaGcGCUCCAGC-GCUCUGa-GCGCC- -5' |
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23201 | 5' | -56.8 | NC_005259.1 | + | 28961 | 0.75 | 0.290969 |
Target: 5'- gCAUCGcCGAGGUCGgugcCGAGGC-CGCGa -3' miRNA: 3'- gGUAGC-GCUCCAGC----GCUCUGaGCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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