Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23202 | 3' | -56 | NC_005259.1 | + | 53251 | 0.66 | 0.791709 |
Target: 5'- gCCGACACCgUCGccgCCGcuguCGCcGGUg -3' miRNA: 3'- gGGCUGUGG-AGCuaaGGCu---GCGaCCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 20847 | 0.66 | 0.791709 |
Target: 5'- gUCCGACGCCUgcaaGGUgUUCGACGCgaucaagcaGGUg -3' miRNA: 3'- -GGGCUGUGGAg---CUA-AGGCUGCGa--------CCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 16654 | 0.66 | 0.776405 |
Target: 5'- gCCGACaauuucggggugaccGCCUCGAUguggUCGGCGaucCUGGg -3' miRNA: 3'- gGGCUG---------------UGGAGCUAa---GGCUGC---GACCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 8069 | 0.66 | 0.772522 |
Target: 5'- gCCGACAUCgagaUCGug-CCG-CGCUGGc -3' miRNA: 3'- gGGCUGUGG----AGCuaaGGCuGCGACCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 13449 | 0.66 | 0.76272 |
Target: 5'- -gCGugACCgUCGGcg-CGGCGCUGGUg -3' miRNA: 3'- ggGCugUGG-AGCUaagGCUGCGACCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 25964 | 0.67 | 0.732607 |
Target: 5'- gCCGACGCCUgCGAUgagaCCGggcaGCGCcgaGGUg -3' miRNA: 3'- gGGCUGUGGA-GCUAa---GGC----UGCGa--CCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 45605 | 0.67 | 0.722367 |
Target: 5'- aCCGACAgCUCGGUcUCGAUGCccgccGGg -3' miRNA: 3'- gGGCUGUgGAGCUAaGGCUGCGa----CCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 58744 | 0.67 | 0.701647 |
Target: 5'- aCCGGCACCggCGAagUUCggcgaaCGACGCUGcGg -3' miRNA: 3'- gGGCUGUGGa-GCU--AAG------GCUGCGAC-Ca -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 10894 | 0.69 | 0.617027 |
Target: 5'- aCCCGGCcCCgUCG-UUCCucGAUGCUGGc -3' miRNA: 3'- -GGGCUGuGG-AGCuAAGG--CUGCGACCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 19081 | 0.69 | 0.602169 |
Target: 5'- gUCCGGCGCUacgacaagcugcgCGAacucaCCGGCGCUGGUg -3' miRNA: 3'- -GGGCUGUGGa------------GCUaa---GGCUGCGACCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 52140 | 0.69 | 0.585247 |
Target: 5'- gUCCGGCACCUCG---CCGAgGCaGGc -3' miRNA: 3'- -GGGCUGUGGAGCuaaGGCUgCGaCCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 9819 | 0.7 | 0.512696 |
Target: 5'- gCCCG-CACCgcCGggUgCGGCGCUGGc -3' miRNA: 3'- -GGGCuGUGGa-GCuaAgGCUGCGACCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 11957 | 0.71 | 0.453604 |
Target: 5'- gCCCGgcgaGCACgUCGAacCCGGCGaCUGGUa -3' miRNA: 3'- -GGGC----UGUGgAGCUaaGGCUGC-GACCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 32222 | 0.72 | 0.425474 |
Target: 5'- aCCGACGCCUCGGcaCCGA-GCUGc- -3' miRNA: 3'- gGGCUGUGGAGCUaaGGCUgCGACca -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 26386 | 0.72 | 0.425474 |
Target: 5'- gCCUGuuGCUcgCGAUUCCGACGCcGGg -3' miRNA: 3'- -GGGCugUGGa-GCUAAGGCUGCGaCCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 50770 | 0.75 | 0.309109 |
Target: 5'- -aCGACACCUCGGccUCG-CGCUGGUa -3' miRNA: 3'- ggGCUGUGGAGCUaaGGCuGCGACCA- -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 63668 | 0.78 | 0.177809 |
Target: 5'- gCCgGACACCUCGGgcaCCGGCGCgccgGGg -3' miRNA: 3'- -GGgCUGUGGAGCUaa-GGCUGCGa---CCa -5' |
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23202 | 3' | -56 | NC_005259.1 | + | 60450 | 1.08 | 0.001523 |
Target: 5'- gCCCGACACCUCGAUUCCGACGCUGGUc -3' miRNA: 3'- -GGGCUGUGGAGCUAAGGCUGCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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