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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23203 | 5' | -56.6 | NC_005259.1 | + | 62725 | 0.69 | 0.542078 |
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Target: 5'- gGGAUCUCGC---UCGaCCGGAcCGGCGGc -3' miRNA: 3'- -UUUAGGGCGguuAGC-GGCCU-GUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 25192 | 0.69 | 0.53165 |
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Target: 5'- ----gCCGCCGAUCccaCCGGGguCAGCGGc -3' miRNA: 3'- uuuagGGCGGUUAGc--GGCCU--GUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 18770 | 0.69 | 0.521296 |
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Target: 5'- ---gCCUGCCAcAUCGCggUGGGCAaGCGGu -3' miRNA: 3'- uuuaGGGCGGU-UAGCG--GCCUGU-CGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 3286 | 0.69 | 0.511021 |
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Target: 5'- -cGUCgCCGCC--UCGCCGGucccuCGGCGa -3' miRNA: 3'- uuUAG-GGCGGuuAGCGGCCu----GUCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 7779 | 0.69 | 0.511021 |
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Target: 5'- ---cCCCGCCGAgCGCCc--CGGCGGg -3' miRNA: 3'- uuuaGGGCGGUUaGCGGccuGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 7025 | 0.69 | 0.511021 |
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Target: 5'- ---cCCCGCCGAgCGCCc--CGGCGGg -3' miRNA: 3'- uuuaGGGCGGUUaGCGGccuGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 28224 | 0.69 | 0.490733 |
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Target: 5'- -cGUgCCGCCAc-CGUCGG-CGGCGGg -3' miRNA: 3'- uuUAgGGCGGUuaGCGGCCuGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 37049 | 0.7 | 0.470827 |
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Target: 5'- ---gCCCGCCAc-CGCCcacGGCGGCGGu -3' miRNA: 3'- uuuaGGGCGGUuaGCGGc--CUGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 43028 | 0.7 | 0.461028 |
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Target: 5'- cGAUgCCGCCGAgcaggcCGCCGaGCAGCGa -3' miRNA: 3'- uUUAgGGCGGUUa-----GCGGCcUGUCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 60372 | 1.07 | 0.001289 |
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Target: 5'- gAAAUCCCGCCAAUCGCCGGACAGCGGc -3' miRNA: 3'- -UUUAGGGCGGUUAGCGGCCUGUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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