Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23204 | 3' | -57.5 | NC_005259.1 | + | 59564 | 1.11 | 0.000552 |
Target: 5'- aACUACUGCCCUCCUCUACGCAGCCGCu -3' miRNA: 3'- -UGAUGACGGGAGGAGAUGCGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 30378 | 0.73 | 0.280063 |
Target: 5'- --cGCUGCCCggcgagaaaccgggCCUCUACcguagcCAGCCGCa -3' miRNA: 3'- ugaUGACGGGa-------------GGAGAUGc-----GUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 28150 | 0.72 | 0.305859 |
Target: 5'- --aGCUGCCCggCCUCgACGC-GCUGCc -3' miRNA: 3'- ugaUGACGGGa-GGAGaUGCGuCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 46371 | 0.72 | 0.320961 |
Target: 5'- gGC-ACcGCCCggccCCaUCUcACGCAGCCGCg -3' miRNA: 3'- -UGaUGaCGGGa---GG-AGA-UGCGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 29914 | 0.7 | 0.378134 |
Target: 5'- --gGCUGCCCgagaCCaUCgacGCGUGGCCGCc -3' miRNA: 3'- ugaUGACGGGa---GG-AGa--UGCGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 22829 | 0.7 | 0.42296 |
Target: 5'- gACUGCgucgugcugGCCCggCCcaccCGCAGCCGCa -3' miRNA: 3'- -UGAUGa--------CGGGa-GGagauGCGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 56999 | 0.69 | 0.432301 |
Target: 5'- --aGCUGCCCgugcgagcaCCUCaGCGCGGCUcgGCg -3' miRNA: 3'- ugaUGACGGGa--------GGAGaUGCGUCGG--CG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 17554 | 0.69 | 0.449414 |
Target: 5'- uCUACuucaccgUGCCCgacgagaUCCUCccgACGCuGCCGCg -3' miRNA: 3'- uGAUG-------ACGGG-------AGGAGa--UGCGuCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 17396 | 0.69 | 0.470827 |
Target: 5'- cACUGCcGCCCagCUacagagguUCUACGaGGCCGCa -3' miRNA: 3'- -UGAUGaCGGGa-GG--------AGAUGCgUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 62917 | 0.68 | 0.521296 |
Target: 5'- uACUAC-GCUCgUCCUCgguuUGCGGCuCGCg -3' miRNA: 3'- -UGAUGaCGGG-AGGAGau--GCGUCG-GCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 48524 | 0.67 | 0.563127 |
Target: 5'- cGC-ACUGCCgaCUUCUCgGCGCuGUCGCc -3' miRNA: 3'- -UGaUGACGG--GAGGAGaUGCGuCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 47574 | 0.67 | 0.584388 |
Target: 5'- cGCUGCUGuCCCUCg---GCGguGuuGCc -3' miRNA: 3'- -UGAUGAC-GGGAGgagaUGCguCggCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 39635 | 0.67 | 0.583321 |
Target: 5'- --cGCUGCCCgguuuguUCCgucCUACGguGUCGUc -3' miRNA: 3'- ugaUGACGGG-------AGGa--GAUGCguCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 63702 | 0.67 | 0.582254 |
Target: 5'- uGCgcCUGCCCagCggggaucggggcCUGCGCAGCCGg -3' miRNA: 3'- -UGauGACGGGagGa-----------GAUGCGUCGGCg -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 45332 | 0.67 | 0.563127 |
Target: 5'- aACUGCgugGCCUgcgCCgcCUGgGCcGCCGCg -3' miRNA: 3'- -UGAUGa--CGGGa--GGa-GAUgCGuCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 8352 | 0.67 | 0.563127 |
Target: 5'- cGCUGCUGCCgguaUCgCUCgGCGgCGGCCucgGCa -3' miRNA: 3'- -UGAUGACGGg---AG-GAGaUGC-GUCGG---CG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 63413 | 0.67 | 0.552572 |
Target: 5'- ---gUUGCCCUUgCUCaUGCcgaGCAGCCGCu -3' miRNA: 3'- ugauGACGGGAG-GAG-AUG---CGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 63142 | 0.66 | 0.627301 |
Target: 5'- aGCcGCUGUCCUCgCccacgUUGaGCAGCCGCc -3' miRNA: 3'- -UGaUGACGGGAG-Ga----GAUgCGUCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 18828 | 0.66 | 0.638062 |
Target: 5'- uGCUGuauCUGCCCggugagggUCUCgcCGguGCCGUg -3' miRNA: 3'- -UGAU---GACGGGa-------GGAGauGCguCGGCG- -5' |
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23204 | 3' | -57.5 | NC_005259.1 | + | 784 | 0.66 | 0.638062 |
Target: 5'- gGCgGCUGCCCUCggUUGgGUGGCCa- -3' miRNA: 3'- -UGaUGACGGGAGgaGAUgCGUCGGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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