miRNA: 3'- -UGAUGaCGGGa-GG--------AGAUGCgUCGGCG- -5'
miRNA: 3'- -UGAUGACGGg---AG-GAGaUGC-GUCGG---CG- -5'
miRNA: 3'- -UGaUGACGGGAGgaGAUgCGUCGGcg -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23204 | 3' | -57.5 | NC_005259.1 | + | 17396 | 0.69 | 0.470827 |
|
Target: 5'- cACUGCcGCCCagCUacagagguUCUACGaGGCCGCa -3' miRNA: 3'- -UGAUGaCGGGa-GG--------AGAUGCgUCGGCG- -5' |
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| 23204 | 3' | -57.5 | NC_005259.1 | + | 8352 | 0.67 | 0.563127 |
|
Target: 5'- cGCUGCUGCCgguaUCgCUCgGCGgCGGCCucgGCa -3' miRNA: 3'- -UGAUGACGGg---AG-GAGaUGC-GUCGG---CG- -5' |
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| 23204 | 3' | -57.5 | NC_005259.1 | + | 784 | 0.66 | 0.638062 |
|
Target: 5'- gGCgGCUGCCCUCggUUGgGUGGCCa- -3' miRNA: 3'- -UGaUGACGGGAGgaGAUgCGUCGGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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