Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23204 | 5' | -60.4 | NC_005259.1 | + | 23648 | 0.7 | 0.286888 |
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Target: 5'- --uCGG-CCCGCCGACGGcGAC-GGCu -3' miRNA: 3'- aguGCCaGGGCGGCUGUC-CUGuCCGu -5' |
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| 23204 | 5' | -60.4 | NC_005259.1 | + | 18341 | 0.71 | 0.266902 |
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Target: 5'- uUCGUGGUCgaCGCCGACGGcGACAuguGGCAc -3' miRNA: 3'- -AGUGCCAGg-GCGGCUGUC-CUGU---CCGU- -5' |
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| 23204 | 5' | -60.4 | NC_005259.1 | + | 58504 | 0.71 | 0.254214 |
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Target: 5'- cUCGCGGUCUaggGUCGACAcgaccuGACGGGCAa -3' miRNA: 3'- -AGUGCCAGGg--CGGCUGUc-----CUGUCCGU- -5' |
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| 23204 | 5' | -60.4 | NC_005259.1 | + | 7057 | 0.72 | 0.242026 |
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Target: 5'- gCGCGaGaUCCUGUCGACGGG-CGGGCu -3' miRNA: 3'- aGUGC-C-AGGGCGGCUGUCCuGUCCGu -5' |
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| 23204 | 5' | -60.4 | NC_005259.1 | + | 8944 | 0.73 | 0.193097 |
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Target: 5'- aUCACGGUgggCgCGCCGu--GGACGGGCAc -3' miRNA: 3'- -AGUGCCA---GgGCGGCuguCCUGUCCGU- -5' |
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| 23204 | 5' | -60.4 | NC_005259.1 | + | 59602 | 1.08 | 0.000548 |
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Target: 5'- cUCACGGUCCCGCCGACAGGACAGGCAc -3' miRNA: 3'- -AGUGCCAGGGCGGCUGUCCUGUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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