Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23208 | 5' | -55.9 | NC_005259.1 | + | 67158 | 0.73 | 0.371235 |
Target: 5'- cUUGUCGAugGUGGcGGCCAUGccgccgaugaUGCCCGCCu -3' miRNA: 3'- -AGUAGCU--CGCC-UUGGUAC----------ACGGGCGG- -5' |
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23208 | 5' | -55.9 | NC_005259.1 | + | 65155 | 0.73 | 0.338204 |
Target: 5'- -aGUCGAaaCGG-GCCAUG-GCCCGCCg -3' miRNA: 3'- agUAGCUc-GCCuUGGUACaCGGGCGG- -5' |
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23208 | 5' | -55.9 | NC_005259.1 | + | 27400 | 0.74 | 0.330287 |
Target: 5'- -uGUCGgcagcGGCGGcAGCUcgGUGCCCGCg -3' miRNA: 3'- agUAGC-----UCGCC-UUGGuaCACGGGCGg -5' |
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23208 | 5' | -55.9 | NC_005259.1 | + | 36317 | 0.74 | 0.292051 |
Target: 5'- ---gCGGGCGGGAUCAUGUuggcaaacgaaauGCCCGCg -3' miRNA: 3'- aguaGCUCGCCUUGGUACA-------------CGGGCGg -5' |
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23208 | 5' | -55.9 | NC_005259.1 | + | 53624 | 0.75 | 0.271896 |
Target: 5'- gCGUCGAGCGGuGCCGU-UGgCCGCUu -3' miRNA: 3'- aGUAGCUCGCCuUGGUAcACgGGCGG- -5' |
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23208 | 5' | -55.9 | NC_005259.1 | + | 57627 | 1.13 | 0.000665 |
Target: 5'- cUCAUCGAGCGGAACCAUGUGCCCGCCg -3' miRNA: 3'- -AGUAGCUCGCCUUGGUACACGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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