miRNA: 3'- -CGGUAGGAa-CGCGACU--AGCaGga-GCG- -5'
miRNA: 3'- -CGguaGGAACGCGAcUAGC---------AGGAGCG- -5'
miRNA: 3'- -CGg-UAGGAa---CGCGACuaGCAGGAGcg -5'
miRNA: 3'- -CGgUAGGAA-CGCGACUAGCA-----GGAGCG- -5'
miRNA: 3'- -CGGUAGGAACGCGACUAGCAGGAGCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23210 | 5' | -54.2 | NC_005259.1 | + | 17024 | 0.73 | 0.462546 |
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Target: 5'- cGCCAUCCUcgGCGCUGGcaUCGaCaacCGCg -3' miRNA: 3'- -CGGUAGGAa-CGCGACU--AGCaGga-GCG- -5' |
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| 23210 | 5' | -54.2 | NC_005259.1 | + | 68258 | 0.73 | 0.424007 |
|
Target: 5'- cGCag-CCUUGCGCUcGUCGuaggcaccgUCCUCGCc -3' miRNA: 3'- -CGguaGGAACGCGAcUAGC---------AGGAGCG- -5' |
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| 23210 | 5' | -54.2 | NC_005259.1 | + | 59035 | 0.74 | 0.387454 |
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Target: 5'- aGCagAUCCUugagGCGCUGcgCGUCCUCa- -3' miRNA: 3'- -CGg-UAGGAa---CGCGACuaGCAGGAGcg -5' |
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| 23210 | 5' | -54.2 | NC_005259.1 | + | 40958 | 0.81 | 0.14335 |
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Target: 5'- aGUgAUCCUUgGCGCUGAUCGUcaugcCCUUGCg -3' miRNA: 3'- -CGgUAGGAA-CGCGACUAGCA-----GGAGCG- -5' |
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| 23210 | 5' | -54.2 | NC_005259.1 | + | 57103 | 1.13 | 0.000902 |
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Target: 5'- cGCCAUCCUUGCGCUGAUCGUCCUCGCc -3' miRNA: 3'- -CGGUAGGAACGCGACUAGCAGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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