Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23214 | 3' | -58 | NC_005259.1 | + | 58933 | 0.66 | 0.679881 |
Target: 5'- uCGGCGGGCGCGgugGCAGcGUCGgCCu- -3' miRNA: 3'- -GCUGCUUGCGCa--UGUCcCGGUgGGca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 24784 | 0.66 | 0.679881 |
Target: 5'- cCGcCGuAUGCGcaGCAGGGCCAagaCCGa -3' miRNA: 3'- -GCuGCuUGCGCa-UGUCCCGGUg--GGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 41355 | 0.66 | 0.669402 |
Target: 5'- aCGACGAACucccagcacaGCGUGCGGucgagcuuGGCCgguACCCa- -3' miRNA: 3'- -GCUGCUUG----------CGCAUGUC--------CCGG---UGGGca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 60178 | 0.66 | 0.668353 |
Target: 5'- gCGGCGAACGCcUGCucgaccgaccGGGCCACgaucucgCCGa -3' miRNA: 3'- -GCUGCUUGCGcAUGu---------CCCGGUG-------GGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 13811 | 0.66 | 0.648358 |
Target: 5'- cCGGCGAgcaccACGUGUGCGugaugaccgacGGGCacauuGCCCGg -3' miRNA: 3'- -GCUGCU-----UGCGCAUGU-----------CCCGg----UGGGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 27999 | 0.66 | 0.648358 |
Target: 5'- -uGCGggUGCcgggGCGGGGUCGCCgGg -3' miRNA: 3'- gcUGCuuGCGca--UGUCCCGGUGGgCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 8388 | 0.66 | 0.648358 |
Target: 5'- uCGGCGAGagccucgGCGaGCcGGGCCACCuCGc -3' miRNA: 3'- -GCUGCUUg------CGCaUGuCCCGGUGG-GCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 45326 | 0.66 | 0.62726 |
Target: 5'- cCGcCGAACuGCGUGgccugcgccgcCuGGGCCGCCgCGUa -3' miRNA: 3'- -GCuGCUUG-CGCAU-----------GuCCCGGUGG-GCA- -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 31078 | 0.66 | 0.624095 |
Target: 5'- uCGGCGAgguucccgaGCGCGU-CGGGGaucaguccgaugagUCGCCCGa -3' miRNA: 3'- -GCUGCU---------UGCGCAuGUCCC--------------GGUGGGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 58761 | 0.67 | 0.585176 |
Target: 5'- uCGGCGAACGaCGcUGCGGGcGCUguGCgCCGg -3' miRNA: 3'- -GCUGCUUGC-GC-AUGUCC-CGG--UG-GGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 66003 | 0.67 | 0.585176 |
Target: 5'- uCGACGugguGCGCacccuCGGGGCCGCaCGUg -3' miRNA: 3'- -GCUGCu---UGCGcau--GUCCCGGUGgGCA- -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 43953 | 0.68 | 0.52326 |
Target: 5'- uCGGCGAugG---GCGGGGCCGCCg-- -3' miRNA: 3'- -GCUGCUugCgcaUGUCCCGGUGGgca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 14325 | 0.68 | 0.513168 |
Target: 5'- uCGACGccgccgccCGCGUGC-GGGCCGCCg-- -3' miRNA: 3'- -GCUGCuu------GCGCAUGuCCCGGUGGgca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 1341 | 0.69 | 0.493233 |
Target: 5'- aGAuCGAGCGCaacgGUGCcuacuGGGCCGCUCGc -3' miRNA: 3'- gCU-GCUUGCG----CAUGu----CCCGGUGGGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 8666 | 0.69 | 0.483399 |
Target: 5'- gGGCugccACGCGggcaACGGGGCCGCCuCGa -3' miRNA: 3'- gCUGcu--UGCGCa---UGUCCCGGUGG-GCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 33105 | 0.69 | 0.473661 |
Target: 5'- -cGCGAGCGUGccUGUAGGGCCACgCGg -3' miRNA: 3'- gcUGCUUGCGC--AUGUCCCGGUGgGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 44317 | 0.69 | 0.463063 |
Target: 5'- uGACGAGCGUGUugAGcGCCGCguagcugCCGg -3' miRNA: 3'- gCUGCUUGCGCAugUCcCGGUG-------GGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 26515 | 0.69 | 0.454486 |
Target: 5'- cCGGCGAgaagaGCGCGgugaGCAGcuuGGCCACCaCGa -3' miRNA: 3'- -GCUGCU-----UGCGCa---UGUC---CCGGUGG-GCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 49986 | 0.7 | 0.435739 |
Target: 5'- gCGcACGGAuCGCGUGCGGacGGCUGCCCu- -3' miRNA: 3'- -GC-UGCUU-GCGCAUGUC--CCGGUGGGca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 64615 | 0.7 | 0.417447 |
Target: 5'- aCGGCGAGCGCG-ACGGGcaGCagCGCCCa- -3' miRNA: 3'- -GCUGCUUGCGCaUGUCC--CG--GUGGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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