Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23214 | 3' | -58 | NC_005259.1 | + | 8388 | 0.66 | 0.648358 |
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Target: 5'- uCGGCGAGagccucgGCGaGCcGGGCCACCuCGc -3' miRNA: 3'- -GCUGCUUg------CGCaUGuCCCGGUGG-GCa -5' |
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| 23214 | 3' | -58 | NC_005259.1 | + | 60178 | 0.66 | 0.668353 |
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Target: 5'- gCGGCGAACGCcUGCucgaccgaccGGGCCACgaucucgCCGa -3' miRNA: 3'- -GCUGCUUGCGcAUGu---------CCCGGUG-------GGCa -5' |
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| 23214 | 3' | -58 | NC_005259.1 | + | 41355 | 0.66 | 0.669402 |
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Target: 5'- aCGACGAACucccagcacaGCGUGCGGucgagcuuGGCCgguACCCa- -3' miRNA: 3'- -GCUGCUUG----------CGCAUGUC--------CCGG---UGGGca -5' |
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| 23214 | 3' | -58 | NC_005259.1 | + | 24784 | 0.66 | 0.679881 |
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Target: 5'- cCGcCGuAUGCGcaGCAGGGCCAagaCCGa -3' miRNA: 3'- -GCuGCuUGCGCa-UGUCCCGGUg--GGCa -5' |
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| 23214 | 3' | -58 | NC_005259.1 | + | 58933 | 0.66 | 0.679881 |
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Target: 5'- uCGGCGGGCGCGgugGCAGcGUCGgCCu- -3' miRNA: 3'- -GCUGCUUGCGCa--UGUCcCGGUgGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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