Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23214 | 5' | -52.3 | NC_005259.1 | + | 17207 | 0.72 | 0.606763 |
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Target: 5'- -gGCGgGUAGAcgggacUGGGCGCGAGucaaauCGCCAg -3' miRNA: 3'- caUGCgCAUCU------ACUCGCGCUU------GUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 36287 | 0.73 | 0.551826 |
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Target: 5'- -aGCGCGauuuucuUGAGCGCGAGCGCUu -3' miRNA: 3'- caUGCGCaucu---ACUCGCGCUUGUGGu -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 8267 | 0.73 | 0.498487 |
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Target: 5'- -cGCGaCGUAGGUGuuguaccggguGCGCGGGCACCc -3' miRNA: 3'- caUGC-GCAUCUACu----------CGCGCUUGUGGu -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 66924 | 0.74 | 0.447573 |
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Target: 5'- -cGCGCGgugcucgaccgGGGUGAGCG-GGACACCGg -3' miRNA: 3'- caUGCGCa----------UCUACUCGCgCUUGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 47058 | 0.78 | 0.270065 |
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Target: 5'- -aGCGUGUuGGUGAGCgagGCGAGCGCCGa -3' miRNA: 3'- caUGCGCAuCUACUCG---CGCUUGUGGU- -5' |
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| 23214 | 5' | -52.3 | NC_005259.1 | + | 55478 | 1.09 | 0.002587 |
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Target: 5'- uGUACGCGUAGAUGAGCGCGAACACCAg -3' miRNA: 3'- -CAUGCGCAUCUACUCGCGCUUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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