Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23215 | 5' | -56.3 | NC_005259.1 | + | 58642 | 0.66 | 0.725251 |
Target: 5'- aGUGCCCAUGcCCUcgcgguagcccgGUGcacCGGGGUgCa -3' miRNA: 3'- cUACGGGUGCuGGA------------UACa--GCCCCAgG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 67226 | 0.66 | 0.714877 |
Target: 5'- cGAUGCcgaCCACGGCg-GUGUaGGGGUUg -3' miRNA: 3'- -CUACG---GGUGCUGgaUACAgCCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 60581 | 0.66 | 0.704426 |
Target: 5'- gGcgGCCCACGGCga--GUCGGGuG-CCg -3' miRNA: 3'- -CuaCGGGUGCUGgauaCAGCCC-CaGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 67187 | 0.66 | 0.702327 |
Target: 5'- uGAUGCCCGCcucgucucccucGCCgcUGUCggcGGGGUCg -3' miRNA: 3'- -CUACGGGUGc-----------UGGauACAG---CCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 40880 | 0.67 | 0.693908 |
Target: 5'- --aGCUCAcCGGCCaccGUGgCGGGGUCg -3' miRNA: 3'- cuaCGGGU-GCUGGa--UACaGCCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 23261 | 0.67 | 0.683334 |
Target: 5'- cGGUGgCCACcaugagcaccaaGACCgggcgcgGUGguuggguaUCGGGGUCCg -3' miRNA: 3'- -CUACgGGUG------------CUGGa------UAC--------AGCCCCAGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 1020 | 0.67 | 0.672714 |
Target: 5'- uAUGCCCuACGGCaaaacaCUGUGcCGGguacGGUCCg -3' miRNA: 3'- cUACGGG-UGCUG------GAUACaGCC----CCAGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 49286 | 0.68 | 0.640675 |
Target: 5'- --cGUCUACGuCgUAgucgacaucGUCGGGGUCCa -3' miRNA: 3'- cuaCGGGUGCuGgAUa--------CAGCCCCAGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 56750 | 0.68 | 0.608567 |
Target: 5'- cGAUGaCCCACGugUggcccaUCGGGGaCCg -3' miRNA: 3'- -CUAC-GGGUGCugGauac--AGCCCCaGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 63289 | 0.68 | 0.587245 |
Target: 5'- cGAUGCCuuCACGugCgagcagCGGGGUCa -3' miRNA: 3'- -CUACGG--GUGCugGauaca-GCCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 46518 | 0.7 | 0.503949 |
Target: 5'- cGAU-CCCGCGAgCCg--GUCGGGGaucgCCg -3' miRNA: 3'- -CUAcGGGUGCU-GGauaCAGCCCCa---GG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 20334 | 0.7 | 0.474 |
Target: 5'- --cGUgCACGACCUAUGgCGGGcgguacuacgcGUCCa -3' miRNA: 3'- cuaCGgGUGCUGGAUACaGCCC-----------CAGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 57195 | 0.71 | 0.448784 |
Target: 5'- --gGCCCGCGaACCUcgacgccguugaaaaAccgguUGUCGGGGUCg -3' miRNA: 3'- cuaCGGGUGC-UGGA---------------U-----ACAGCCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 55885 | 0.71 | 0.444971 |
Target: 5'- --cGCCC-CGACCUcgGgggucgcgcCGGGGUCg -3' miRNA: 3'- cuaCGGGuGCUGGAuaCa--------GCCCCAGg -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 40686 | 0.74 | 0.294355 |
Target: 5'- uGGUGCUCAcCGGCCcgAUGaUCGGGGUgCCc -3' miRNA: 3'- -CUACGGGU-GCUGGa-UAC-AGCCCCA-GG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 60719 | 0.82 | 0.084941 |
Target: 5'- uGUGUCCGaauaucCGGCCcGUGUCGGGGUCCa -3' miRNA: 3'- cUACGGGU------GCUGGaUACAGCCCCAGG- -5' |
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23215 | 5' | -56.3 | NC_005259.1 | + | 55238 | 1.12 | 0.000648 |
Target: 5'- cGAUGCCCACGACCUAUGUCGGGGUCCa -3' miRNA: 3'- -CUACGGGUGCUGGAUACAGCCCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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