Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23216 | 3' | -50.5 | NC_005259.1 | + | 26613 | 0.72 | 0.781662 |
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Target: 5'- gCCGCGccggacCGGUAGCcGAUAucGAUGGCCu -3' miRNA: 3'- -GGCGCuu----GCCAUCGcUUAU--CUGCUGG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 45791 | 0.72 | 0.761583 |
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Target: 5'- aCCGCGuuGACGGUGuccugcuuggguGCGuagccggGGAUGACCa -3' miRNA: 3'- -GGCGC--UUGCCAU------------CGCuua----UCUGCUGG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 61014 | 0.73 | 0.719937 |
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Target: 5'- aCgGUGAGCuuccacucGGUGGCGGcgAGGuCGACCg -3' miRNA: 3'- -GgCGCUUG--------CCAUCGCUuaUCU-GCUGG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 47053 | 0.73 | 0.719937 |
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Target: 5'- gCGCGAGCGuGuUGGUGAGcgAGGCGAgCg -3' miRNA: 3'- gGCGCUUGC-C-AUCGCUUa-UCUGCUgG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 26438 | 0.73 | 0.698552 |
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Target: 5'- aCCGcCGAGCGG--GCGGGccGGugGGCCg -3' miRNA: 3'- -GGC-GCUUGCCauCGCUUa-UCugCUGG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 18377 | 0.84 | 0.219206 |
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Target: 5'- cCCGaGGGCGGUAGCGAGggAGACGAgCa -3' miRNA: 3'- -GGCgCUUGCCAUCGCUUa-UCUGCUgG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 27919 | 0.86 | 0.150485 |
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Target: 5'- gCGCGggUGGUAGCGGuucGGCGACCc -3' miRNA: 3'- gGCGCuuGCCAUCGCUuauCUGCUGG- -5' |
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| 23216 | 3' | -50.5 | NC_005259.1 | + | 54892 | 1.15 | 0.002108 |
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Target: 5'- aCCGCGAACGGUAGCGAAUAGACGACCu -3' miRNA: 3'- -GGCGCUUGCCAUCGCUUAUCUGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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