Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23216 | 5' | -56.2 | NC_005259.1 | + | 51822 | 0.66 | 0.693402 |
Target: 5'- cAGGGCgggCAg--ACCCGCCCgGUUg-- -3' miRNA: 3'- -UCUCGa--GUacaUGGGCGGGgCAGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 6055 | 0.66 | 0.692324 |
Target: 5'- cGAGCUCGUGUGCgcggugaUCGCCgCCGa---- -3' miRNA: 3'- uCUCGAGUACAUG-------GGCGG-GGCagaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 25940 | 0.67 | 0.64997 |
Target: 5'- uGAGCgcacgCcgGUGCCCGgCCCGcCg-- -3' miRNA: 3'- uCUCGa----GuaCAUGGGCgGGGCaGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 36711 | 0.67 | 0.628116 |
Target: 5'- uGAGCUCGUGccgcucgACCCGCCgCGcCc-- -3' miRNA: 3'- uCUCGAGUACa------UGGGCGGgGCaGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 67172 | 0.67 | 0.606275 |
Target: 5'- -cGGC-CAUGccgccgaugaUGCCCGCCUCGUCUc- -3' miRNA: 3'- ucUCGaGUAC----------AUGGGCGGGGCAGAaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 57632 | 0.67 | 0.601915 |
Target: 5'- cGAGCggaacCAUGUGCCCGCCggguacagcagcaCGUCg-- -3' miRNA: 3'- uCUCGa----GUACAUGGGCGGg------------GCAGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 42980 | 0.67 | 0.595383 |
Target: 5'- cGGGGaUCAUcGUugCCGCCCCGcCg-- -3' miRNA: 3'- -UCUCgAGUA-CAugGGCGGGGCaGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 20800 | 0.68 | 0.5737 |
Target: 5'- aAGAGC-CAccUGCUCGUCCCGUCg-- -3' miRNA: 3'- -UCUCGaGUacAUGGGCGGGGCAGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 31751 | 0.69 | 0.520448 |
Target: 5'- gAGcGCUgGUGUccccaCCGCCCCGUCa-- -3' miRNA: 3'- -UCuCGAgUACAug---GGCGGGGCAGaaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 40141 | 0.75 | 0.238461 |
Target: 5'- cGAGCUCGccgucgguugucgGUGCCCGCCCCGgcagCUc- -3' miRNA: 3'- uCUCGAGUa------------CAUGGGCGGGGCa---GAaa -5' |
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23216 | 5' | -56.2 | NC_005259.1 | + | 54926 | 1.06 | 0.00152 |
Target: 5'- cAGAGCUCAUGUACCCGCCCCGUCUUUg -3' miRNA: 3'- -UCUCGAGUACAUGGGCGGGGCAGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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