miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23218 3' -46.6 NC_005259.1 + 54275 1.13 0.006648
Target:  5'- uCCGUUUCCCGAGACUGAAAAUGACACu -3'
miRNA:   3'- -GGCAAAGGGCUCUGACUUUUACUGUG- -5'
23218 3' -46.6 NC_005259.1 + 41614 0.69 0.982036
Target:  5'- uUCGgaUCgCGAGGCUGAccauGGUGGCGg -3'
miRNA:   3'- -GGCaaAGgGCUCUGACUu---UUACUGUg -5'
23218 3' -46.6 NC_005259.1 + 35202 0.68 0.98787
Target:  5'- aCCGUgccgCCUGAGGCggcgagGAAcGUGAC-Cg -3'
miRNA:   3'- -GGCAaa--GGGCUCUGa-----CUUuUACUGuG- -5'
23218 3' -46.6 NC_005259.1 + 13964 0.67 0.99509
Target:  5'- cCCGgcaUCCCGAGGCccgcgaugcuUGAGAucuggGACAa -3'
miRNA:   3'- -GGCaa-AGGGCUCUG----------ACUUUua---CUGUg -5'
23218 3' -46.6 NC_005259.1 + 44444 0.66 0.996508
Target:  5'- cCCGaagaugUUCuuGAGACcGggGAUGcCGCc -3'
miRNA:   3'- -GGCa-----AAGggCUCUGaCuuUUACuGUG- -5'
23218 3' -46.6 NC_005259.1 + 57266 0.66 0.997991
Target:  5'- uCCGUUgggCgaGGGAUUGAccGGUGGCACc -3'
miRNA:   3'- -GGCAAa--GggCUCUGACUu-UUACUGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.