Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23218 | 5' | -55.8 | NC_005259.1 | + | 28079 | 0.66 | 0.707843 |
Target: 5'- aCACGGUggggugCACCUCGaagugcagauguGGGCCGg -3' miRNA: 3'- cGUGCCGauaaa-GUGGAGC------------CCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 15552 | 0.66 | 0.729015 |
Target: 5'- gGC-CGGUgccacccUCAgguggccacCCUCGGGGCCAg -3' miRNA: 3'- -CGuGCCGauaa---AGU---------GGAGCCCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 13135 | 0.66 | 0.729015 |
Target: 5'- cGCACGGUg--UUCGgCUCGaucauGGCCAc -3' miRNA: 3'- -CGUGCCGauaAAGUgGAGCc----CCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 28361 | 0.66 | 0.739463 |
Target: 5'- gGUGCuGGCUGcccCACCUCG-GGCCGa -3' miRNA: 3'- -CGUG-CCGAUaaaGUGGAGCcCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 15142 | 0.67 | 0.63201 |
Target: 5'- uGUACGGggc---CACCUCGGuGGCCGa -3' miRNA: 3'- -CGUGCCgauaaaGUGGAGCC-CCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 32878 | 0.67 | 0.653827 |
Target: 5'- cGCGCGGUgaucgaUUCACCg-GGGGCgAg -3' miRNA: 3'- -CGUGCCGaua---AAGUGGagCCCCGgUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 50628 | 0.67 | 0.653827 |
Target: 5'- aGCACaGCgguccaugcgAUUaCGCCgcccUCGGGGCCAUa -3' miRNA: 3'- -CGUGcCGa---------UAAaGUGG----AGCCCCGGUA- -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 57020 | 0.67 | 0.664711 |
Target: 5'- aGCGCGGCUcgg-CGCggUGGGGUCGc -3' miRNA: 3'- -CGUGCCGAuaaaGUGgaGCCCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 31932 | 0.67 | 0.686379 |
Target: 5'- cGCACucggggucgaGGCgg--UUACCUCGGGuGCCu- -3' miRNA: 3'- -CGUG----------CCGauaaAGUGGAGCCC-CGGua -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 64766 | 0.68 | 0.577628 |
Target: 5'- cGCgACGGUg----CGCCUCGGcGGCCc- -3' miRNA: 3'- -CG-UGCCGauaaaGUGGAGCC-CCGGua -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 34008 | 0.68 | 0.610192 |
Target: 5'- uGCAacCGGCUGUaUCcgGCCUCGGcGGCg-- -3' miRNA: 3'- -CGU--GCCGAUAaAG--UGGAGCC-CCGgua -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 18702 | 0.7 | 0.463059 |
Target: 5'- aGCACGGCgGUcUgACCUCGaucaucGGGCCGc -3' miRNA: 3'- -CGUGCCGaUAaAgUGGAGC------CCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 68205 | 0.7 | 0.473021 |
Target: 5'- aGCAUGGCgugcUUCugCUCGGuGGaCCAc -3' miRNA: 3'- -CGUGCCGaua-AAGugGAGCC-CC-GGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 16796 | 0.71 | 0.414992 |
Target: 5'- aCGCGGUcgcgUAcaUCGCgCUCGGGGCCGg -3' miRNA: 3'- cGUGCCG----AUaaAGUG-GAGCCCCGGUa -5' |
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23218 | 5' | -55.8 | NC_005259.1 | + | 54315 | 1.07 | 0.001461 |
Target: 5'- uGCACGGCUAUUUCACCUCGGGGCCAUc -3' miRNA: 3'- -CGUGCCGAUAAAGUGGAGCCCCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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