Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23219 | 5' | -60.1 | NC_005259.1 | + | 20976 | 0.66 | 0.547464 |
Target: 5'- cGCCGGGGUGacaaCUGCGUgugggccuacucgGGCgGCGAc -3' miRNA: 3'- cUGGCCCCGCcaa-GACGCA-------------UCG-CGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 27387 | 0.66 | 0.538277 |
Target: 5'- uGACCGGcGGCGGUgUCgGC--AGCgGCGGc -3' miRNA: 3'- -CUGGCC-CCGCCA-AGaCGcaUCG-CGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 7207 | 0.66 | 0.517058 |
Target: 5'- cACCGGGcuGCGGUggaUCUGCGUgaugaacgacggcAGcCGCGc -3' miRNA: 3'- cUGGCCC--CGCCA---AGACGCA-------------UC-GCGCu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 35896 | 0.67 | 0.488327 |
Target: 5'- aGACCacGGCGaGccaUUCUGCGUuGCGCGGg -3' miRNA: 3'- -CUGGccCCGC-C---AAGACGCAuCGCGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 13432 | 0.67 | 0.467999 |
Target: 5'- cGCCGGGGCcGcgCUagGCGUgaccgucGGCGCGGc -3' miRNA: 3'- cUGGCCCCGcCaaGA--CGCA-------UCGCGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 47040 | 0.68 | 0.422348 |
Target: 5'- aGGCCGGGGa---UCUGCGcgAGCGUGu -3' miRNA: 3'- -CUGGCCCCgccaAGACGCa-UCGCGCu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 41629 | 0.68 | 0.387133 |
Target: 5'- uGACCauGGuGGCGGUgccggUCUGCG-GGCGCu- -3' miRNA: 3'- -CUGG--CC-CCGCCA-----AGACGCaUCGCGcu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 26834 | 0.69 | 0.362022 |
Target: 5'- uGCCGGGGCGG-UCUGCucgGGCa--- -3' miRNA: 3'- cUGGCCCCGCCaAGACGca-UCGcgcu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 4299 | 0.69 | 0.353907 |
Target: 5'- gGACCGGcGGCc---CUGCGaugAGCGCGAc -3' miRNA: 3'- -CUGGCC-CCGccaaGACGCa--UCGCGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 28005 | 0.72 | 0.235828 |
Target: 5'- uGCCGGGGCGGggUCgccgGgGUuGCGCGc -3' miRNA: 3'- cUGGCCCCGCCa-AGa---CgCAuCGCGCu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 23282 | 0.73 | 0.213341 |
Target: 5'- aGACCGGGcGCGGUggUUGgGUaucgggguccGGCGCGGu -3' miRNA: 3'- -CUGGCCC-CGCCAa-GACgCA----------UCGCGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 1558 | 0.75 | 0.137356 |
Target: 5'- -uUCGGGGCGGUgacgCUGUG-AGCGCGc -3' miRNA: 3'- cuGGCCCCGCCAa---GACGCaUCGCGCu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 49198 | 0.76 | 0.117071 |
Target: 5'- gGACCGGGGCGGUUUc-CGUuGUGCGGg -3' miRNA: 3'- -CUGGCCCCGCCAAGacGCAuCGCGCU- -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 35311 | 0.76 | 0.117071 |
Target: 5'- uGACCGaGGGCGGUguguacgCgaccccGCGUAGCGCGu -3' miRNA: 3'- -CUGGC-CCCGCCAa------Ga-----CGCAUCGCGCu -5' |
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23219 | 5' | -60.1 | NC_005259.1 | + | 53407 | 1.07 | 0.0006 |
Target: 5'- gGACCGGGGCGGUUCUGCGUAGCGCGAc -3' miRNA: 3'- -CUGGCCCCGCCAAGACGCAUCGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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