Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23220 | 3' | -47.2 | NC_005259.1 | + | 13917 | 0.66 | 0.994279 |
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Target: 5'- cCACAGAaAGGAAccGAAccAUGACCGcCAa -3' miRNA: 3'- aGUGUCUcUCCUU--CUU--UACUGGUaGU- -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 6855 | 0.66 | 0.993289 |
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Target: 5'- cUACAGAGAGaaGAucGAAAUGGCCcaccUCAa -3' miRNA: 3'- aGUGUCUCUC--CUu-CUUUACUGGu---AGU- -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 16276 | 0.66 | 0.992166 |
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Target: 5'- aUCGCAauGGcuGAAGguAUGACCAUCAa -3' miRNA: 3'- -AGUGUc-UCucCUUCuuUACUGGUAGU- -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 29955 | 0.66 | 0.990899 |
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Target: 5'- gUCGCGGcugguGGGGcGGGcgGUGACCGUCc -3' miRNA: 3'- -AGUGUCu----CUCC-UUCuuUACUGGUAGu -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 48807 | 0.68 | 0.979602 |
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Target: 5'- cCGCcGAGgaAGGuAGAAAUGACCAccaUCAc -3' miRNA: 3'- aGUGuCUC--UCCuUCUUUACUGGU---AGU- -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 51957 | 0.74 | 0.801225 |
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Target: 5'- cCGCcgAGAGAGGAAGccAUGACCGcCAg -3' miRNA: 3'- aGUG--UCUCUCCUUCuuUACUGGUaGU- -5' |
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| 23220 | 3' | -47.2 | NC_005259.1 | + | 53129 | 1.11 | 0.006609 |
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Target: 5'- cUCACAGAGAGGAAGAAAUGACCAUCAg -3' miRNA: 3'- -AGUGUCUCUCCUUCUUUACUGGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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