Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23221 | 3' | -58.1 | NC_005259.1 | + | 58202 | 0.67 | 0.577576 |
Target: 5'- gUGGGCUUGcCGGUGUCagGGuCAGUGg- -3' miRNA: 3'- aGCUCGAGCaGCCACAG--CC-GUCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 11133 | 0.66 | 0.666215 |
Target: 5'- gCGAGCcgUCGccugcggguaacacuUCGGUG-CGGCAGUGc- -3' miRNA: 3'- aGCUCG--AGC---------------AGCCACaGCCGUCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 44162 | 0.66 | 0.662 |
Target: 5'- gUCGAGCagcgCGcCGGUGUCGGagaaaAGuCGa- -3' miRNA: 3'- -AGCUCGa---GCaGCCACAGCCg----UC-GCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 51631 | 0.66 | 0.651443 |
Target: 5'- aCGAGgUCGUCGGag-CGGUAGgCGa- -3' miRNA: 3'- aGCUCgAGCAGCCacaGCCGUC-GCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 37961 | 0.66 | 0.651443 |
Target: 5'- uUCGGGCUC-UUGG--UCGGCAGUGc- -3' miRNA: 3'- -AGCUCGAGcAGCCacAGCCGUCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 50407 | 0.66 | 0.640869 |
Target: 5'- cCGAGCgCGcCGGUGccuugaccggcUCgGGCAGCGg- -3' miRNA: 3'- aGCUCGaGCaGCCAC-----------AG-CCGUCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 52630 | 0.66 | 0.640869 |
Target: 5'- gUCGAGCUgCGUCGGcUGUgGGgcgaGGUGa- -3' miRNA: 3'- -AGCUCGA-GCAGCC-ACAgCCg---UCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 24249 | 0.66 | 0.640869 |
Target: 5'- gCGAGUUCGgCGaGUG-CGuGCAcGCGUGg -3' miRNA: 3'- aGCUCGAGCaGC-CACaGC-CGU-CGCAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 53605 | 0.66 | 0.640869 |
Target: 5'- ---uGCUCGUCGGgGUCaGCAGgCGUc -3' miRNA: 3'- agcuCGAGCAGCCaCAGcCGUC-GCAc -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 58971 | 0.66 | 0.609141 |
Target: 5'- gUCGggcAGCUCGUCGGcGaCGGCgAGCuUGg -3' miRNA: 3'- -AGC---UCGAGCAGCCaCaGCCG-UCGcAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 51792 | 0.66 | 0.609141 |
Target: 5'- uUCGGcGCUCGgCGG-GUCGGuCAGCu-- -3' miRNA: 3'- -AGCU-CGAGCaGCCaCAGCC-GUCGcac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 57311 | 0.67 | 0.59859 |
Target: 5'- aUCGAGCacCGgCGGUGUCGGU-GUGg- -3' miRNA: 3'- -AGCUCGa-GCaGCCACAGCCGuCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 41846 | 0.67 | 0.588066 |
Target: 5'- cUCGAcCUCGaCGGUGUCccacucgacgGGCAGCaccGUGg -3' miRNA: 3'- -AGCUcGAGCaGCCACAG----------CCGUCG---CAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 60284 | 0.67 | 0.587016 |
Target: 5'- cCGGGCggccaucgUCGUCGGUG-CGGCcacggacGGCGa- -3' miRNA: 3'- aGCUCG--------AGCAGCCACaGCCG-------UCGCac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 52765 | 1.08 | 0.000891 |
Target: 5'- aUCGAGCUCGUCGGUGUCGGCAGCGUGu -3' miRNA: 3'- -AGCUCGAGCAGCCACAGCCGUCGCAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 9729 | 0.68 | 0.536102 |
Target: 5'- gUCGAGCUCGcCGaUGUucCGGUcauccaccacAGCGUGa -3' miRNA: 3'- -AGCUCGAGCaGCcACA--GCCG----------UCGCAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 51346 | 0.68 | 0.525888 |
Target: 5'- gUGAGCUCGgauaugccCGGUGgcacCGGCAcaccgcGCGUGu -3' miRNA: 3'- aGCUCGAGCa-------GCCACa---GCCGU------CGCAC- -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 40140 | 0.69 | 0.485831 |
Target: 5'- gCGAGCUCGccgucgguugUCGGUGcccgcccCGGCAGCu-- -3' miRNA: 3'- aGCUCGAGC----------AGCCACa------GCCGUCGcac -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 10435 | 0.69 | 0.456763 |
Target: 5'- gCGAGCUCGacaUCGaGUGgcucgaCGGCGGUGUc -3' miRNA: 3'- aGCUCGAGC---AGC-CACa-----GCCGUCGCAc -5' |
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23221 | 3' | -58.1 | NC_005259.1 | + | 59127 | 0.71 | 0.36725 |
Target: 5'- aUCGAGCagagCGUCGagcUCGGCAGCGg- -3' miRNA: 3'- -AGCUCGa---GCAGCcacAGCCGUCGCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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