Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23222 | 3' | -51.4 | NC_005259.1 | + | 29880 | 0.66 | 0.916904 |
Target: 5'- cCGcCCGAUgucgAGGUCGGCAAGCa---- -3' miRNA: 3'- uGCuGGUUG----UCUAGCCGUUCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 27386 | 0.66 | 0.916904 |
Target: 5'- gUGACCGGCGGcgguGUCGGCAgcGGCg---- -3' miRNA: 3'- uGCUGGUUGUC----UAGCCGU--UCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 40886 | 0.66 | 0.910382 |
Target: 5'- cCGGCCAccgugGCgGGGUCGGCAGcgucccacGCUUGGu -3' miRNA: 3'- uGCUGGU-----UG-UCUAGCCGUU--------CGAACUu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 63614 | 0.66 | 0.90357 |
Target: 5'- gGCGGCCAccgacgccugAUAGAUCaucguGGCGAGCUg--- -3' miRNA: 3'- -UGCUGGU----------UGUCUAG-----CCGUUCGAacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 28999 | 0.67 | 0.881428 |
Target: 5'- uACGGCCAcgAGAUCGGCGAauGCg---- -3' miRNA: 3'- -UGCUGGUugUCUAGCCGUU--CGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 42551 | 0.67 | 0.848134 |
Target: 5'- gUGGCgGGCAGcUCGGCGAGCa---- -3' miRNA: 3'- uGCUGgUUGUCuAGCCGUUCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 67834 | 0.68 | 0.839188 |
Target: 5'- uCGACCGGCAGccCGGUGAGCg---- -3' miRNA: 3'- uGCUGGUUGUCuaGCCGUUCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 32509 | 0.68 | 0.82061 |
Target: 5'- aAC-ACCGugAGGUagucgaggcgggCGGCAGGCUUGAc -3' miRNA: 3'- -UGcUGGUugUCUA------------GCCGUUCGAACUu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 56243 | 0.68 | 0.811 |
Target: 5'- cCGACCAcguugGGAUCGGCGGcCUUGAGc -3' miRNA: 3'- uGCUGGUug---UCUAGCCGUUcGAACUU- -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 62995 | 0.68 | 0.811 |
Target: 5'- uCGACuCGAUGGcAUUGGCAGGCUgugGGAa -3' miRNA: 3'- uGCUG-GUUGUC-UAGCCGUUCGAa--CUU- -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 44556 | 0.68 | 0.809054 |
Target: 5'- -aGACCGGCAGGcagcgcgccgacCGGCAGGCUcaUGAu -3' miRNA: 3'- ugCUGGUUGUCUa-----------GCCGUUCGA--ACUu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 41791 | 0.68 | 0.801191 |
Target: 5'- uCGGCgAGCAG-UCGGCAGGCg---- -3' miRNA: 3'- uGCUGgUUGUCuAGCCGUUCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 49714 | 0.69 | 0.791194 |
Target: 5'- -aGAuCCGGCAGAuauUCGGCGGGCggcGAGa -3' miRNA: 3'- ugCU-GGUUGUCU---AGCCGUUCGaa-CUU- -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 25441 | 0.7 | 0.727939 |
Target: 5'- uGCGAgCCguAugAGGUCGGCGAGCgucuUGAc -3' miRNA: 3'- -UGCU-GG--UugUCUAGCCGUUCGa---ACUu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 58960 | 0.71 | 0.672314 |
Target: 5'- uUGGCCAGCAGGucgggcagcucgUCGGCGacggcgAGCUUGGc -3' miRNA: 3'- uGCUGGUUGUCU------------AGCCGU------UCGAACUu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 3541 | 0.71 | 0.66102 |
Target: 5'- cCGACCAcagcCAGAUCcGCGAGCUUGc- -3' miRNA: 3'- uGCUGGUu---GUCUAGcCGUUCGAACuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 5731 | 0.72 | 0.615671 |
Target: 5'- gGCGGCCAuucucGCGGccaagCGGCGAGCUgccUGAAa -3' miRNA: 3'- -UGCUGGU-----UGUCua---GCCGUUCGA---ACUU- -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 67698 | 0.75 | 0.412689 |
Target: 5'- aAUGAUCAugAGAUCGGCGGGCa---- -3' miRNA: 3'- -UGCUGGUugUCUAGCCGUUCGaacuu -5' |
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23222 | 3' | -51.4 | NC_005259.1 | + | 52507 | 1.06 | 0.004321 |
Target: 5'- uACGACCAACAGAUCGGCAAGCUUGAAu -3' miRNA: 3'- -UGCUGGUUGUCUAGCCGUUCGAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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