miRNA: 3'- -CGGGguUC-UAACCuaCaCGCUCGGGC- -5'
miRNA: 3'- -CGGGGUUCua-ACCuACAcGCUCGGGC- -5'
miRNA: 3'- -CGGGGUuCUaaCCUAC-ACGCuCGGgc -5'
miRNA: 3'- -CGGGGUUCUAACCUACACGCUCGGGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23222 | 5' | -55.5 | NC_005259.1 | + | 40122 | 0.67 | 0.696205 |
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Target: 5'- cGCUCguGGaAUUGGcgG-GCGAGCUCGc -3' miRNA: 3'- -CGGGguUC-UAACCuaCaCGCUCGGGC- -5' |
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| 23222 | 5' | -55.5 | NC_005259.1 | + | 48078 | 0.67 | 0.674789 |
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Target: 5'- cGCCCUGAGcgaUGG-UGU-UGAGCCCGa -3' miRNA: 3'- -CGGGGUUCua-ACCuACAcGCUCGGGC- -5' |
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| 23222 | 5' | -55.5 | NC_005259.1 | + | 27540 | 0.69 | 0.599124 |
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Target: 5'- gGCCCCAcGGcgGGAUGuUGCG-GCCa- -3' miRNA: 3'- -CGGGGUuCUaaCCUAC-ACGCuCGGgc -5' |
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| 23222 | 5' | -55.5 | NC_005259.1 | + | 52541 | 1.12 | 0.000828 |
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Target: 5'- cGCCCCAAGAUUGGAUGUGCGAGCCCGa -3' miRNA: 3'- -CGGGGUUCUAACCUACACGCUCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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