Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23226 | 3' | -60.8 | NC_005259.1 | + | 60251 | 0.66 | 0.503695 |
Target: 5'- uGCGUGCGGGugcaaGGCUGgCCaCcaGCGCu -3' miRNA: 3'- -CGCACGCCUg----CCGACgGGaGcaCGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 62845 | 0.66 | 0.503695 |
Target: 5'- aGCGcGCGGuugACGaGCaGCCgCUCG-GCGCg -3' miRNA: 3'- -CGCaCGCC---UGC-CGaCGG-GAGCaCGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 2038 | 0.66 | 0.484075 |
Target: 5'- -aGUGCGGugGGCgGUCaC-CG-GCACc -3' miRNA: 3'- cgCACGCCugCCGaCGG-GaGCaCGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 8226 | 0.66 | 0.455356 |
Target: 5'- aGCGUGCGGugGuGCcgacacccUGCCUUacCGgucuuggGCGCg -3' miRNA: 3'- -CGCACGCCugC-CG--------ACGGGA--GCa------CGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 50505 | 0.67 | 0.445988 |
Target: 5'- uCGaUGUGGGCGGUgcgcUGCaCCUCGacGCGCu -3' miRNA: 3'- cGC-ACGCCUGCCG----ACG-GGAGCa-CGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 14644 | 0.67 | 0.418549 |
Target: 5'- uGCGUGaCGauggcGugGGCUcaGCCCgacucgggccgCGUGCACu -3' miRNA: 3'- -CGCAC-GC-----CugCCGA--CGGGa----------GCACGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 63861 | 0.68 | 0.383608 |
Target: 5'- gGCGagGUGGcCGGCUGgCCUugCGUGgGCg -3' miRNA: 3'- -CGCa-CGCCuGCCGACgGGA--GCACgUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 16315 | 0.69 | 0.334955 |
Target: 5'- uGUGaUGUGGGCGGauaCUGCCCga-UGCACg -3' miRNA: 3'- -CGC-ACGCCUGCC---GACGGGagcACGUG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 767 | 0.7 | 0.264239 |
Target: 5'- cGCGUccaccGCGccgaGGCGGCUGCCCUCGguuggGUg- -3' miRNA: 3'- -CGCA-----CGC----CUGCCGACGGGAGCa----CGug -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 22891 | 0.73 | 0.171875 |
Target: 5'- cGCGcUGCGucuguacGACGGCaagcaggaUGCCCUCGUGCuCg -3' miRNA: 3'- -CGC-ACGC-------CUGCCG--------ACGGGAGCACGuG- -5' |
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23226 | 3' | -60.8 | NC_005259.1 | + | 49996 | 1.11 | 0.000256 |
Target: 5'- cGCGUGCGGACGGCUGCCCUCGUGCACg -3' miRNA: 3'- -CGCACGCCUGCCGACGGGAGCACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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