Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23229 | 3' | -55.3 | NC_005259.1 | + | 3418 | 0.66 | 0.788351 |
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Target: 5'- cGGGAuCGaCCCGCCGAGaAAGGaucGAAa -3' miRNA: 3'- -CCUU-GCaGGGCGGCUCcUUCCau-CUU- -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 5636 | 0.66 | 0.7687 |
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Target: 5'- cGGAuCGUgCCCGgCGAGGAcGGcGGGc -3' miRNA: 3'- -CCUuGCA-GGGCgGCUCCUuCCaUCUu -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 9088 | 0.69 | 0.599124 |
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Target: 5'- aGGccCGUugCCCGCCGAGGgcGG-GGAAc -3' miRNA: 3'- -CCuuGCA--GGGCGGCUCCuuCCaUCUU- -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 2435 | 0.74 | 0.330272 |
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Target: 5'- cGGGACgGUCCCGCCGAGaAAGGa---- -3' miRNA: 3'- -CCUUG-CAGGGCGGCUCcUUCCaucuu -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 51947 | 0.75 | 0.299773 |
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Target: 5'- cGGGACaGUCCCGCCGAGaGAGGa---- -3' miRNA: 3'- -CCUUG-CAGGGCGGCUCcUUCCaucuu -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 28514 | 0.77 | 0.21021 |
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Target: 5'- cGGGAuCGaCCCGCCGAGGAAGGa---- -3' miRNA: 3'- -CCUU-GCaGGGCGGCUCCUUCCaucuu -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 79 | 0.89 | 0.036261 |
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Target: 5'- cGGGAcCGUCCCGCCGAGGAAGGggucAGAc -3' miRNA: 3'- -CCUU-GCAGGGCGGCUCCUUCCa---UCUu -5' |
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| 23229 | 3' | -55.3 | NC_005259.1 | + | 48798 | 1.08 | 0.001536 |
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Target: 5'- gGGAACGUCCCGCCGAGGAAGGUAGAAa -3' miRNA: 3'- -CCUUGCAGGGCGGCUCCUUCCAUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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